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RecombinantRabMAb

Recombinant Anti-Cyclin E2 antibody [E142] (ab32103)

Reviews (5)Q&A (3)References (22)
Western blot - Anti-Cyclin E2 antibody [E142] (ab32103)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)
  • Western blot - Anti-Cyclin E2 antibody [E142] (ab32103)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)
  • Anti-Cyclin E2 antibody [E142] (ab32103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E142] to Cyclin E2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

Overview

  • Product name

    Anti-Cyclin E2 antibody [E142]
    See all Cyclin E2 primary antibodies

  • Description

    Rabbit monoclonal [E142] to Cyclin E2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human Cyclin E2 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O96020

  • Positive control

    • Human breast carcinoma, HeLa cells lysates, Jurkat whole cell lysate (ab7899)

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/200. Predicted molecular weight: 45 kDa.

For unpurified version use at 1/500
ICC/IF (1)
1/50.

For unpurified use at 1/100 - 1/250
Notes
WB
1/200. Predicted molecular weight: 45 kDa.

For unpurified version use at 1/500
ICC/IF
1/50.

For unpurified use at 1/100 - 1/250

Target

  • Relevance

    The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • CCN E2 antibody
      • CCNE 2 antibody
      • CCNE2 antibody
      • CCNE2 protein antibody
      • CYC E2 antibody
      • CYCE 2 antibody
      • CYCE2 antibody
      • CyclinE2 antibody
      • G1/S specific cyclin E2 antibody
      see all

    Images

    • Western blot - Anti-Cyclin E2 antibody [E142] (ab32103)
      Western blot - Anti-Cyclin E2 antibody [E142] (ab32103)
      All lanes : Anti-Cyclin E2 antibody [E142] (ab32103) at 1/200 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : Mouse testis lysate
      Lane 3 : Mouse thymus lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)

      Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin E2 with purified ab32103 at 1/50 dilution (5.2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Western blot - Anti-Cyclin E2 antibody [E142] (ab32103)
      Western blot - Anti-Cyclin E2 antibody [E142] (ab32103)
      Anti-Cyclin E2 antibody [E142] (ab32103) at 1/500 dilution + HeLa cell lysate

      Predicted band size: 45 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)

      Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling Cyclin E2 with purified ab32103 at 1/2000. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)
      Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 ab32103.
    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E2 antibody [E142] (ab32103)This image is courtesy of an abreview by Kirk Mcmanus

      ab32103 staining Cyclin E2 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.

      See Abreview

    • Anti-Cyclin E2 antibody [E142] (ab32103)
      Anti-Cyclin E2 antibody [E142] (ab32103)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (22)

    Publishing research using ab32103? Please let us know so that we can cite the reference in this datasheet.

    ab32103 has been referenced in 22 publications.

    • Li Y  et al. miRNA-128 modulates bone neoplasms cells proliferation and migration through the WNT/ß-catenin and EMT signal pathways. J Orthop Surg Res 16:71 (2021). PubMed: 33472642
    • Liang Y  et al. Exosomal microRNA-144 from bone marrow-derived mesenchymal stem cells inhibits the progression of non-small cell lung cancer by targeting CCNE1 and CCNE2. Stem Cell Res Ther 11:87 (2020). PubMed: 32102682
    • Wu D  et al. CARM1 promotes non-small cell lung cancer progression through upregulating CCNE2 expression. Aging (Albany NY) 12:10578-10593 (2020). PubMed: 32487779
    • Wang W  et al. DJ-1 is a new prognostic marker and predicts chemotherapy efficacy in colorectal cancer. Oncol Rep 44:77-90 (2020). PubMed: 32627002
    • Wei L  et al. A Small Molecule Inhibitor, OGP46, Is Effective against Imatinib-Resistant BCR-ABL Mutations via the BCR-ABL/JAK-STAT Pathway. Mol Ther Oncolytics 18:137-148 (2020). PubMed: 32671189
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin E2 antibody [E142]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    Submitted Aug 25 2017

    Western blot abreview for Anti-Cyclin E2 antibody [E142]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Liver)
    Loading amount
    50 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 28 2011

    Western blot abreview for Anti-Cyclin E2 antibody [E142]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Hepa 1-6)
    Loading amount
    50 µg
    Specification
    Hepa 1-6
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 23 2011

    Western blot abreview for Anti-Cyclin E2 antibody [E142]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2)
    Loading amount
    50 µg
    Specification
    HepG2
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mrs. Sadaf Sultan

    Verified customer

    Submitted Aug 11 2011

    Western blot abreview for Anti-Cyclin E2 antibody [E142]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cell line)
    Loading amount
    50 µg
    Specification
    HeLa cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Shakil Ahmad

    Verified customer

    Submitted Aug 11 2011

    Question

    IHC-P and chromosome spreads
    received warm and tried it, but no signal
    worked with same Ab before and it was fine
    mouse prostate tissue
    AR: Tris pH 9
    Ab: 1/100 o/n
    block: donkey serum
    2nd: OK

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. The estimated delivery date is xxx.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Question

    BATCH NUMBER 319539 ORDER NUMBER PO-00843 DESCRIPTION OF THE PROBLEM Cytoplasmic staing and heavy background SAMPLE Breast carcinoma PRIMARY ANTIBODY 1:50 for 2hours DETECTION METHOD Streptavidin HRP label POSITIVE AND NEGATIVE CONTROLS USED Pos-Human Breast carcinoma Neg- Normal rabbit IgG Fraction ANTIBODY STORAGE CONDITIONS 4 degrees FIXATION OF SAMPLE Archived formalin fixed paraffin embedded ANTIGEN RETRIEVAL Pressure cooker and pH 6.0 modified citrate PERMEABILIZATION STEP none BLOCKING CONDITIONS Avidin/Biotin blocks SECONDARY ANTIBODY Biotinylated link HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5+ HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No WHAT STEPS HAVE YOU ALTERED? I have taken over this project from someone else. I have no other information regarding staining parameters ADDITIONAL NOTES I am looking to steer away from the avidin/biotin system and am going to try a pH 9.0 Tris-HCL retrieval in gentle waterbath 20mins and incubate overnight at 4 degrees. There is too much cytoplasmic staining and some stromal background I must get rid of. I just wanted an idea of Abcam’s staining method for IHC so I don’t waste anymore precious tissue or reagent. And time!

    Read More
    Answer

    Thank you for your enquiry. I can confirm that this product does not require any specific protocols and the general IHC-P and antigen retrieval protocols found on our website (can also be accessed on the datasheet) will be able to help you. From the protocols you provided, I agree that you should try incubating at 4oC overnight to ensure proper and sufficient binding to the protein of interest. You can also try increasing the primary antibody dilution to 1:100 ~ 1:1000. The recommended dilution we have on the datasheet is just a guideline and optimal dilutions/concentrations should be determined by the end user. Can I also suggest running a no primary experiment? Sometimes, the problem lies with the secondary antibody and could be binding non-specifically to your sample. I hope the information above will helpful. Please do not hesitate to contact me again if you face problems when using this product.

    Read More

    Question

    I have a customer enquiring about product# ab32103, and want to know the concentration of this antibody. Could you please advise the estimated concentration for this product?

    Read More
    Answer

    Thank you for your enquiry. The approximate concentration of this antibody (ab32103) is 90ug/ml. I hope this is helpful. Please don't hesitate to contact us again if you require more information.

    Read More

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