Recombinant Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)
![Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)](https://www.abcam.com/ps/products/181/ab181983/Images/ab181983-393550-anti-cyclophilin-40-antibody-epr14845-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14845(B)] to Cyclophilin 40
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cyclophilin 40 antibody [EPR14845(B)]
See all Cyclophilin 40 primary antibodies -
Description
Rabbit monoclonal [EPR14845(B)] to Cyclophilin 40 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, JAR, HepG2 and K-562 cell lysate; Human fetal brain lysate; Recombinant Human Cyclophilin 40 protein (BSA and azide free) (ab173064). IHC-P: Human transitional cell carcinoma of bladder. ICC/IF: K562 cell lyaste.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14845(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/10000 - 1/50000. Predicted molecular weight: 41 kDa.
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| ICC/IF |
1/500.
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| Notes |
|---|
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IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/10000 - 1/50000. Predicted molecular weight: 41 kDa. |
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ICC/IF
1/500. |
Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 3 TPR repeats. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5481 Human
- Entrez Gene: 67738 Mouse
- Entrez Gene: 361967 Rat
- Omim: 601753 Human
- SwissProt: Q08752 Human
- SwissProt: Q9CR16 Mouse
- SwissProt: Q6DGG0 Rat
- Unigene: 183958 Human
see all -
Alternative names
- 40 kDa peptidyl prolyl cis trans isomerase antibody
- 40 kDa peptidyl prolyl cis trans isomerase D antibody
- 40 kDa peptidyl-prolyl cis-trans isomerase antibody
see all
Images
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Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)All lanes : Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PPID knockout HeLa cell lysate
Lane 3 : K-562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab181983 observed at 47 kDa. Red - loading control ab8245 observed at 36 kDa.
ab181983 Anti-Cyclophilin 40 antibody [EPR14845(B)] was shown to specifically react with Cyclophilin 40 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265788 (knockout cell lysate ab257600) was used. Wild-type and Cyclophilin 40 knockout samples were subjected to SDS-PAGE. ab181983 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)](https://www.abcam.com/ps/products/181/ab181983/Images/ab181983-216129-anti-cyclophilin-40-antibody-epr14845b-western-blot.jpg)
Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)All lanes : Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983) at 1/20000 dilution
Lane 1 : Human fetal brain
Lane 2 : JAR
Lane 3 : K562
Lane 4 : HepG2
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)](https://www.abcam.com/ps/products/181/ab181983/Images/ab181983-216131-anti-cyclophilin-40-antibody-epr14845b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)Immunofluorescence staining of K562 cells labeling Cyclophilin 40 with ab181983 at 1/200 dilution followed by labeling with Goat
anti rabbit IgG(Alexa Fluor® 555) antibody (1/500 dilution) and counter-staining with Dapi (blue) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)](https://www.abcam.com/ps/products/181/ab181983/Images/ab181983-216130-anti-cyclophilin-40-antibody-epr14845b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)Imunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder labeling Cyclophilin 40 with ab181983 at 1/50 dilution followed by labeing with secondary Ready to use HRP Polymer for Rabbit IgG and hematoxylin staining.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)](https://www.abcam.com/ps/products/181/ab181983/Images/ab181983-5-benefits-of-recombinant-antibodies.png)
Anti-Cyclophilin 40 antibody [EPR14845(B)] (ab181983)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab181983 has been referenced in 3 publications.
- Zeng X et al. Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells. Oncol Lett 21:124 (2021). PubMed: 33552245
- Li J et al. Melatonin attenuates renal fibrosis in diabetic mice by activating the AMPK/PGC1a signaling pathway and rescuing mitochondrial function. Mol Med Rep 19:1318-1330 (2019). PubMed: 30535482
- Zhou H et al. Inhibitory effect of melatonin on necroptosis via repressing the Ripk3-PGAM5-CypD-mPTP pathway attenuates cardiac microvascular ischemia-reperfusion injury. J Pineal Res N/A:e12503 (2018). PubMed: 29770487