Anti-CYP11A1 antibody (ab232763)
Key features and details
- Rabbit polyclonal to CYP11A1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-CYP11A1 antibody
See all CYP11A1 primary antibodies -
Description
Rabbit polyclonal to CYP11A1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Cynomolgus monkey
-
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human CYP11A1 aa 350 to the C-terminus. Expressed in E.coli. N-terminal tags.
Database link: P05108-1 -
Positive control
- WB: Recombinant human CYP11A1 protein; Pig liver and kidney lysates. IHC-P: Human kidney tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
| WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa.
|
| Notes |
|---|
|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa. |
Target
-
Function
Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. -
Pathway
Lipid metabolism; C21-steroid hormone metabolism. -
Involvement in disease
Defects in CYP11A1 are a cause of congenital adrenal insufficiency (CAI).
Defects in CYP11A1 are a cause of congenital lipoid adrenal hyperplasia (CLAH) [MIM:201710]; also known as lipoid CAH. CLAH is the most severe form of adrenal hyperplasia. This autosomal recessive and potentially lethal condition includes the onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. CLAH is a rare disease, except in Japan and Korea where it accounts for a significant percentage of cases of congenital adrenal hyperplasia. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
-
Database links
- Entrez Gene: 102120640 Cynomolgus monkey
- Entrez Gene: 1583 Human
- Entrez Gene: 403329 Pig
- Omim: 118485 Human
- SwissProt: Q2XV99 Cynomolgus monkey
- SwissProt: P05108 Human
- SwissProt: P10612 Pig
- Unigene: 303980 Human
-
Alternative names
- Cholesterol 20 22 desmolase antibody
- Cholesterol desmolase antibody
- Cholesterol monooxygenase (side chain cleaving) antibody
see all
Images
-
Western blot - Anti-CYP11A1 antibody (ab232763)All lanes : Anti-CYP11A1 antibody (ab232763) at 3 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Pig kidney lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP11A1 antibody (ab232763)Formalin-fixed, paraffin-embedded human kidney tissue stained for CYP11A1 using ab232763 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Western blot - Anti-CYP11A1 antibody (ab232763)Anti-CYP11A1 antibody (ab232763) at 3 µg/ml + Recombinant human CYP11A1 protein
Predicted band size: 60 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab232763 has not yet been referenced specifically in any publications.