Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)

Reviews (1) Submit a question References (3)
Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
  • Immunocytochemistry/ Immunofluorescence - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
  • Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
  • Immunoprecipitation - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
  • Flow Cytometry (Intracellular) - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
  • Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
  • Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20681] to CYR61/CCN1
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CYR61/CCN1 antibody [EPR20681]
    See all CYR61/CCN1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20681] to CYR61/CCN1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, PC-3, MDA-MB-231 and Saos-2 whole cell lysate. ICC/IF: MDA-MB-231 cells. Flow Cyt (intra): MDA-MB-231 cells. IP: Saos-2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab230947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60.
WB (1)
1/1000. Predicted molecular weight: 42 kDa.
ICC/IF
1/500.
IP
1/30.
Notes
Flow Cyt (Intra)
1/60.
WB
1/1000. Predicted molecular weight: 42 kDa.
ICC/IF
1/500.
IP
1/30.

Target

  • Function

    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • 3CH61 antibody
      • CCN 1 antibody
      • CCN family member 1 antibody
      • CCN1 antibody
      • CYR 61 antibody
      • Cyr61 antibody
      • CYR61 protein antibody
      • CYR61_HUMAN antibody
      • Cysteine rich angiogenic inducer 61 antibody
      • Cysteine rich heparin binding protein 61 antibody
      • Cysteine-rich angiogenic inducer 61 antibody
      • GIG 1 antibody
      • GIG1 antibody
      • IBP-10 antibody
      • IGF-binding protein 10 antibody
      • Igfbp 10 antibody
      • IGFBP-10 antibody
      • Igfbp10 antibody
      • Insulin like growth factor binding protein 10 antibody
      • Insulin-like growth factor-binding protein 10 antibody
      • Protein CYR61 antibody
      • Protein GIG1 antibody
      see all

    Images

    • Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      All lanes : Anti-CYR61/CCN1 antibody [EPR20681] (ab230947) at 1/500 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : CYR61 knockout HeLa cell lysate
      Lane 3 : MDA-MB-231 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 42 kDa
      Observed band size: 47 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab230947 observed at 47 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab230947 Anti-CYR61/CCN1 antibody [EPR20681] was shown to specifically react with CYR61/CCN1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265288 (knockout cell lysate ab257406) was used.  Wild-type and CYR61/CCN1 knockout samples were subjected to SDS-PAGE.  ab230947 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      Immunocytochemistry/ Immunofluorescence - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labeling CYR61/CCN1 with ab230947 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the MDA-MB-231 cell line. Negative control: MCF7 (PMID: 11059746). The nuclear counter stain is DAPI (blue).
      Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
      The negative control is the secondary antibody only.

    • Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      Anti-CYR61/CCN1 antibody [EPR20681] (ab230947) at 1/5000 dilution + MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa


      Exposure time: 3 minutes


      Blocking/ Dilution buffer concentration: 5% NFDM/TBST.

      The molecular mass observed is consistent with that of the full-length protein (42 kDa) (PMID: 23798676).

    • Immunoprecipitation - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      Immunoprecipitation - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)

      CYR61/CCN1 was immunoprecipitated from 0.35 mg Saos-2 (human osteosarcoma epithelial) whole cell lysate with ab230947 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230947 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
      Lane 1: Saos-2 (human osteosarcoma epithelial) whole cell lysate 10 µg (Input).
      Lane 2: ab230947 IP in Saos-2 whole cell lysate (+).
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230947 in Saos-2 whole cell lysate (-).
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 8 seconds.

      The molecular mass observed is consistent with that of the full-length protein (42 kDa) (PMID: 23798676).

    • Flow Cytometry (Intracellular) - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      Flow Cytometry (Intracellular) - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell, Left) / MDA-MB-231 (human breast adenocarcinoma epithelial cell, Right) cell line labeling CYR61/CCN1with ab230947 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor� 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

      Negative control: MCF7 (PMID: 11059746). 

       

       

    • Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      All lanes : Anti-CYR61/CCN1 antibody [EPR20681] (ab230947) at 1/1000 dilution

      Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : Saos-2 (human osteosarcoma epithelial) whole cell lysate
      Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 42 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer concentration: 5% NFDM/TBST.

      The expression profile observed is consistent with the literature, with the 42 kDa band being the full-length protein and the 19 kDa band likely a degradation product (PMID: 23798676; PMID: 16266990; PMID: 8657105).

      Negative control: MCF7 (PMID 11059746).

    • Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)
      Anti-CYR61/CCN1 antibody [EPR20681] (ab230947)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab230947? Please let us know so that we can cite the reference in this datasheet.

    ab230947 has been referenced in 3 publications.

    • Geng H  et al. HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway. Mol Med Rep 24:N/A (2021). PubMed: 34396425
    • Gao F  et al. ARID1A-mutated cervical cancer depends on the activation of YAP signaling. Front Biosci (Landmark Ed) 26:1411-1421 (2021). PubMed: 34994156
    • Li Z  et al. Transplantation of human endometrial perivascular cells with elevated CYR61 expression induces angiogenesis and promotes repair of a full-thickness uterine injury in rat. Stem Cell Res Ther 10:179 (2019). PubMed: 31215503

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Western blot abreview for Anti-CYR61/CCN1 antibody [EPR20681]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast cancer cell lines)
    Gel Running Conditions
    Reduced Denaturing (10 % running gel)
    Loading amount
    20 µg
    Specification
    Breast cancer cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 03 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com