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Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)

Submit a review Q&A (1)References (8)
Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
  • Flow Cytometry - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)

Key features and details

  • Mouse monoclonal [3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Rat, Cow, Human
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488

Overview

  • Product name

    Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3]
    See all Cytochrome C Oxidase subunit VIc/COX6C primary antibodies

  • Description

    Mouse monoclonal [3G5F7G3] to Cytochrome C Oxidase subunit VIc/COX6C

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Rat, Cow, Human

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart, bovine heart, and rat heart. IF/ICC: HeLa cell line

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 9 kDa.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 9 kDa.

Target

  • Function

    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

  • Sequence similarities

    Belongs to the cytochrome c oxidase subunit 6c family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • COX 6C antibody
      • Cox6c antibody
      • COX6C_HUMAN antibody
      • Cytochrome c oxidase polypeptide VIc antibody
      • Cytochrome c oxidase subunit 6C antibody
      • cytochrome c oxidase subunit VIc antibody
      • Cytochrome c oxidase subunit VIc preprotein antibody
      see all

    Images

    • Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
      Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
      All lanes : Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267) at 2 µg/ml

      Lane 1 : Isolated mitochondria from Human heart at 50 µg
      Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
      Lane 3 : Isolated mitochondria from Rat heart at 10 µg

      Predicted band size: 9 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)

      ICC/IF image of ab110267 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
      Flow Cytometry - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [3G5F7G3] (ab110267)
      Overlay histogram showing HepG2 cells stained with ab110267 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110267, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References (8)

    Publishing research using ab110267? Please let us know so that we can cite the reference in this datasheet.

    ab110267 has been referenced in 8 publications.

    • Pajuelo Reguera D  et al. Cytochrome c Oxidase Subunit 4 Isoform Exchange Results in Modulation of Oxygen Affinity. Cells 9:N/A (2020). PubMed: 32075102
    • Chang FW  et al. Estrogen Enhances the Expression of the Multidrug Transporter Gene ABCG2-Increasing Drug Resistance of Breast Cancer Cells through Estrogen Receptors. Int J Mol Sci 18:N/A (2017). PubMed: 28098816
    • Chen X  et al. Artesunate Activates the Intrinsic Apoptosis of HCT116 Cells through the Suppression of Fatty Acid Synthesis and the NF-?B Pathway. Molecules 22:N/A (2017). PubMed: 28786914
    • Pérez-Pérez R  et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . PubMed: 27545886
    • Sohal RS  et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008). PubMed: 18538373
    • Janssen GM  et al. The A3243G tRNALeu(UUR) mutation induces mitochondrial dysfunction and variable disease expression without dominant negative acting translational defects in complex IV subunits at UUR codons. Hum Mol Genet 16:2472-81 (2007). PubMed: 17656376
    • Amaral A  et al. The expression of polymerase gamma and mitochondrial transcription factor A and the regulation of mitochondrial DNA content in mature human sperm. Hum Reprod 22:1585-96 (2007). PubMed: 17339235
    • Lock RB & Murphy KM Immunodetecting members of the Bcl-2 family of proteins. Methods Mol Med 111:83-96 (2005). PubMed: 15911974

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    We are wondering if the following antibodies can be purchased in a PBS buffer only format:



    ab14734 anti-VDAC1 clone 20B12AF2



    If possible, please provided pricing and lead times.



    Please let me know if there may be any questions.



    ab110267 anti-cytochrome C oxidase clone 3G5F7G3




    Thanks very much for your help.

    Read More
    Answer

    Thank you for contacting Abcam.

    We can look into custom formulation batches if you were interested in ordering 1mg or more of the antibody, but for smaller quantities we do not offer this service.

    If however you only need 1 or 2vials of the antibody, then I would recommend you use one of our purification kits that will allow you to do a buffer exchange, so that you can get the antibody in PBS:

    https://www.abcam.com/Mouse-Antibody-Purification-Kit-ab128745.html



    If there is anything else I can help you with, or if you are interested in a bulk custom order, then please let me know.

    Read More

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