Recombinant Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6294(2)] to Cytochrome P450 17A1/CYP17A1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)]
See all Cytochrome P450 17A1/CYP17A1 primary antibodies -
Description
Rabbit monoclonal [EPR6294(2)] to Cytochrome P450 17A1/CYP17A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human adrenal gland lysate, mouse and rat testis lysate. IHC-P: Human and mouse testis tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6294(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 57 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. -
Pathway
Lipid metabolism; steroid biosynthesis. -
Involvement in disease
Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic). -
Sequence similarities
Belongs to the cytochrome P450 family. -
Post-translational
modificationsPhosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1586 Human
- Entrez Gene: 13074 Mouse
- Entrez Gene: 25146 Rat
- Omim: 609300 Human
- SwissProt: P05093 Human
- SwissProt: P27786 Mouse
- SwissProt: P11715 Rat
- Unigene: 438016 Human
see all -
Alternative names
- 20 lyase antibody
- CP17A_HUMAN antibody
- CPT7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Cytochrome P450 17A1/CYP17A1 with ab134910 at 1/250 followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Cytoplasmic staining on mouse Leydig cells.The section was incubated with ab134910 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910) at 1/1000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa -
Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910) at 1/10000 dilution + Human adrenal gland lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Predicted band size: 57 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Cytochrome P450 17A1/CYP17A1 with ab134910 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab134910 has been referenced in 7 publications.
- Long X et al. Obesity modulates cell-cell interactions during ovarian folliculogenesis. iScience 25:103627 (2022). PubMed: 35005562
- Melau C et al. The effects of selected inhibitors on human fetal adrenal steroidogenesis differs under basal and ACTH-stimulated conditions. BMC Med 19:204 (2021). PubMed: 34493283
- Harpelunde Poulsen K et al. Dysregulation of FGFR signalling by a selective inhibitor reduces germ cell survival in human fetal gonads of both sexes and alters the somatic niche in fetal testes. Hum Reprod 34:2228-2243 (2019). PubMed: 31734698
- Melau C et al. Characterization of Human Adrenal Steroidogenesis during Fetal Development. J Clin Endocrinol Metab N/A:N/A (2018). PubMed: 30590593
- Pedroso JAB et al. SOCS3 expression in SF1 cells regulates adrenal differentiation and exercise performance. J Endocrinol 235:207-222 (2017). PubMed: 28899903
- Salvi S et al. CYP17A1 polymorphisms and clinical outcome of castration-resistant prostate cancer patients treated with abiraterone. Int J Biol Markers 31:e264-9 (2016). PubMed: 26954071
- Hofland J et al. Protein kinase C-induced activin A switches adrenocortical steroidogenesis to aldosterone by suppressing CYP17A1 expression. Am J Physiol Endocrinol Metab 305:E736-44 (2013). PubMed: 23900415