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RecombinantRabMAb

Recombinant Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

Reviews (1) Submit a question References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
  • Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6294(2)] to Cytochrome P450 17A1/CYP17A1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)]
    See all Cytochrome P450 17A1/CYP17A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6294(2)] to Cytochrome P450 17A1/CYP17A1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human adrenal gland lysate, mouse and rat testis lysate. IHC-P: Human and mouse testis tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

  • Pathway

    Lipid metabolism; steroid biosynthesis.

  • Involvement in disease

    Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).

  • Sequence similarities

    Belongs to the cytochrome P450 family.

  • Post-translational
    modifications

    Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 20 lyase antibody
    • CP17A_HUMAN antibody
    • CPT7 antibody
    • CYP17 antibody
    • CYP17A1 antibody
    • CYPXVII antibody
    • Cytochrome P450 17A1 antibody
    • Cytochrome P450 family 17 antibody
    • Cytochrome P450 family 17 subfamily A polypeptide 1 antibody
    • Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia antibody
    • Cytochrome p450 XVIIA1 antibody
    • Cytochrome P450-C17 antibody
    • Cytochrome P450c17 antibody
    • OTTHUMP00000020382 antibody
    • P450 C17 antibody
    • P450c17 antibody
    • S17AH antibody
    • Steroid 17 alpha hydroxylase/17,20 lyase antibody
    • Steroid 17 alpha monooxygenase antibody
    • Steroid 17-alpha-hydroxylase/17 antibody
    • Steroid 17-alpha-monooxygenase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Cytochrome P450 17A1/CYP17A1 with ab134910 at 1/250 followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Cytoplasmic staining on mouse Leydig cells.The section was incubated with ab134910 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
    Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
    All lanes : Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 57 kDa

  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
    Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
    Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910) at 1/10000 dilution + Human adrenal gland lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Cytochrome P450 17A1/CYP17A1 with ab134910 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
    Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)

References (5)

Publishing research using ab134910? Please let us know so that we can cite the reference in this datasheet.

ab134910 has been referenced in 5 publications.

  • Harpelunde Poulsen K  et al. Dysregulation of FGFR signalling by a selective inhibitor reduces germ cell survival in human fetal gonads of both sexes and alters the somatic niche in fetal testes. Hum Reprod 34:2228-2243 (2019). PubMed: 31734698
  • Melau C  et al. Characterization of Human Adrenal Steroidogenesis during Fetal Development. J Clin Endocrinol Metab N/A:N/A (2018). PubMed: 30590593
  • Pedroso JAB  et al. SOCS3 expression in SF1 cells regulates adrenal differentiation and exercise performance. J Endocrinol 235:207-222 (2017). PubMed: 28899903
  • Salvi S  et al. CYP17A1 polymorphisms and clinical outcome of castration-resistant prostate cancer patients treated with abiraterone. Int J Biol Markers 31:e264-9 (2016). PubMed: 26954071
  • Hofland J  et al. Protein kinase C-induced activin A switches adrenocortical steroidogenesis to aldosterone by suppressing CYP17A1 expression. Am J Physiol Endocrinol Metab 305:E736-44 (2013). PubMed: 23900415

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)]

 Poor
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 Prostate cancer cell line)
Permeabilization
Yes - 0.1% v/v TritonX-100
Specification
DU145 Prostate cancer cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Jan 23 2017

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