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  1. Link

    products/primary-antibodies/cytokeratin-19-antibody-ep1580y-bsa-and-azide-free-ab195872.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

  • Datasheet
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • OI-RD Scanning - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
  • Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1580Y] to Cytokeratin 19 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC HRP PE Unconjugated

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Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

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Overview

  • Product name

    Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free
    See all Cytokeratin 19 primary antibodies
  • Description

    Rabbit monoclonal [EP1580Y] to Cytokeratin 19 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 cells and HepG2 cells. Flow Cyt (intra): MCF7 and HeLa cells. IHC-P: Mouse salivary gland; Human skin, breast carcinoma, endometrial carcinoma and gastric adenocarcinoma.
  • General notes

    ab195872 is the carrier-free version of ab52625.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 3.70 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1580Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
    • Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192980)
    • HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
    • Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203443)
    • Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444)
    • Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
    • APC Anti-Cytokeratin 19 antibody [EP1580Y] (ab224980)
    • PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
    • Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab195872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.

This product gave a positive signal in MCF7 (-ve: SH-SY5Y) fixed with 100% methanol (5 min).

IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
Use at an assay dependent concentration. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.

This product gave a positive signal in MCF7 (-ve: SH-SY5Y) fixed with 100% methanol (5 min).

IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
Use at an assay dependent concentration. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Application notes
Is unsuitable for IP.

Target

  • Function

    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Present in hair follicles at all stages of development.
  • Domain

    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Target information above from: UniProt accession P08727 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3880 Human
    • Entrez Gene: 16669 Mouse
    • Omim: 148020 Human
    • SwissProt: P08727 Human
    • SwissProt: P19001 Mouse
    • Unigene: 654568 Human
    • Unigene: 439699 Mouse
    • Alternative names

      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Clone EP1580Y (ab195872) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 647). Please refer to ab192980 for protocol details.

      ab192980 staining Cytokeratin 19 in HeLa cells. The cells were fixed with 100% methanol (5 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192980 at a working dilution of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor® 488 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      This product gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions

      Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

    • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      ab52625 staining Cytokeratin 19 in the human cell line MCF-7 (human breast carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody. 

      Isoytype control: Rabbit monoclonal IgG (Black) 

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Clone EP1580Y (ab195872) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 488). Please refer to ab192643 for protocol details.

      ab192643 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192643 at 1/500 dilution (shown in green) and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      This data was developed using ab52625, the same antibody clone in a different buffer formulation. Different batches of ab52625 were tested on HepG2 (Human hepatocellular carcinoma epithelial cell) lysate at 0.2 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 40 kDa.
    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      ab52625 staining KRT19 in MCF7 cells, with negative expression in SHSY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab52625 at 2 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown. This product also work with 4% formaldehyde (10 min) fixation under the same testing conditions.

      This data was developed using the same antibody clone in a different buffer formulation (ab52625).

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      This data was developed using the same antibody clone in a different buffer formulation (ab52625).

      ab52625 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab52625 at 1/100 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Clone EP1580Y (ab195872) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 19 antibody [EP1580Y] (PE). Please refer to ab224981 for protocol details. 

      Overlay histogram showing HeLa cells stained with ab224981 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224981, 1/1000 dilution) for 30 min at 22°C. 

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. 

      Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labelling Cytokeratin 19 (green) with purified ab52625 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by ab150120 AlexaFluor®594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

      For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (ab150077).  

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Immunofluorescent staining of MCF-7 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab52625 at a dilution of 1/200. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200 and the cells were counter stained with DAPI.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Immunohistochemical staining of paraffin-embedded human skin with purified ab52625 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Unpurified ab52625 showing positive staining in Breast carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Unpurified ab52625 showing positive staining in Endometrial carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Unpurified ab52625 showing negative staining in Glioma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Unpurified ab52625 showing positive staining in Gastric adenocarcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

    • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

      Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with unpurified ab52625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52625, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625). 

       

       

    • OI-RD Scanning - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      OI-RD Scanning - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

    • Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
      Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (9)

    Publishing research using ab195872? Please let us know so that we can cite the reference in this datasheet.

    ab195872 has been referenced in 9 publications.

    • Pal B  et al. A single-cell RNA expression atlas of normal, preneoplastic and tumorigenic states in the human breast. EMBO J 40:e107333 (2021). PubMed: 33950524
    • Cui M  et al. PTEN is a potent suppressor of small cell lung cancer. Mol Cancer Res 12:654-9 (2014). PubMed: 24482365
    • Ito S  et al. Cholestasis induces reversible accumulation of periplakin in mouse liver. BMC Gastroenterol 13:116 (2013). PubMed: 23849208
    • Pal SK  et al. The expression profiles of acidic epithelial keratins in ameloblastoma. Oral Surg Oral Med Oral Pathol Oral Radiol 115:523-31 (2013). IHC-P ; Human . PubMed: 23522646
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