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    products/primary-antibodies/cytokeratin-19-antibody-ep1580y-cytoskeleton-marker-ab52625.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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RecombinantRabMAb

Recombinant Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

  • Datasheet
  • SDS
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Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • OI-RD Scanning - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
  • Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1580Y] to Cytokeratin 19 - Cytoskeleton Marker
  • Suitable for: Flow Cyt (Intra), mIHC, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free HRP PE

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Overview

  • Product name

    Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker
    See all Cytokeratin 19 primary antibodies
  • Description

    Rabbit monoclonal [EP1580Y] to Cytokeratin 19 - Cytoskeleton Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), mIHC, ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and NIH/3T3 cell lysates. IHC-P: Human skin, breast carcinoma, kidney carcinoma, endometrial carcinoma and gastric adenocarcinoma tissues. ICC/IF: HepG2 and MCF-7 cells. Flow Cyt (intra): MCF-7 and HeLa cells. IHC-Fr: Mouse salivary gland tissue. mIHC: Hu lung cancer tissue
  • General notes

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Dissociation constant (KD)

    KD = 3.70 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1580Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
    • Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192980)
    • HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
    • Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
    • Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203443)
    • Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444)
    • Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
    • APC Anti-Cytokeratin 19 antibody [EP1580Y] (ab224980)
    • PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30 - 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

mIHC
1/400.
ICC/IF (3)
Use a concentration of 2 µg/ml.

For unpurified, use 1/50. Signal can be observed in cells fixed with either methanol or paraformaldehyde.

This product gave a positive signal in MCF7 (-ve: SH-SY5Y) fixed with 100% methanol (5 min).

WB (1)
1/50000 - 1/200000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

For unpurified, use 1/10000 - 1/50000.

IHC-P (5)
1/400 - 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

For unpurified, use at 1/100.

Notes
Flow Cyt (Intra)
1/30 - 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

mIHC
1/400.
ICC/IF
Use a concentration of 2 µg/ml.

For unpurified, use 1/50. Signal can be observed in cells fixed with either methanol or paraformaldehyde.

This product gave a positive signal in MCF7 (-ve: SH-SY5Y) fixed with 100% methanol (5 min).

WB
1/50000 - 1/200000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

For unpurified, use 1/10000 - 1/50000.

IHC-P
1/400 - 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

For unpurified, use at 1/100.

Application notes
Is unsuitable for IP.

Target

  • Function

    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity

    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Present in hair follicles at all stages of development.
  • Domain

    This keratin differs from all other IF proteins in lacking the C-terminal tail domain.
  • Target information above from: UniProt accession P08727 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3880 Human
    • Entrez Gene: 16669 Mouse
    • Omim: 148020 Human
    • SwissProt: P08727 Human
    • SwissProt: P19001 Mouse
    • Unigene: 654568 Human
    • Unigene: 439699 Mouse
    • Alternative names

      • 40 kDa keratin intermediate filament antibody
      • CK 19 antibody
      • CK-19 antibody
      • CK19 antibody
      • Cytokeratin 19 antibody
      • Cytokeratin-19 antibody
      • K19 antibody
      • K1C19_HUMAN antibody
      • K1CS antibody
      • Keratin 19 antibody
      • Keratin type I 40 kD antibody
      • Keratin type I 40kD antibody
      • Keratin type I cytoskeletal 19 antibody
      • Keratin, type I cytoskeletal 19 antibody
      • Keratin, type I, 40 kd antibody
      • Keratin-19 antibody
      • KRT19 antibody
      • MGC15366 antibody
      see all

    Images

    • Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)This image is courtesy of TissueGnostics Asia Pacific Limited

      10-color fluorescence multiplex immunohistochemical analysis of human lung cancer tissue (formalin-fixed paraffin-embedded section).

      Merged staining of anti-FOXP3 (ab215206; Cyan; TG540N), anti-PD1 (ab52587; Red; TG700N), anti-CD163 (ab182422; Brown; TG650N), anti-HLA-DR (ab92511; Yellow; TG570N), anti-CD4 (ab133616; Violet; TG620N), anti-CD8 alpha (ab101500; Purple; TG540S), anti-CD20 (ab9475; Grey; TG660S), anti-CD68 (ab192847; Green; TG520N), anti-Cytokeratin 19 (ab52625; Light blue; TG440N). TG470SN (dark blue) was used as a nuclear counter stain. The inset image shows the separate Cytokeratin 19 signal.

      The section was incubated in nine rounds of staining; in the order of ab215206 (1/100 dilution), ab52587 (1/200 dilution), ab182422 (1/300 dilution), ab92511 (1/200 dilution), ab133616 (1/600 dilution), ab101500 (1/300 dilution), ab9475 (1/100 dilution), ab192847 (1/300 dilution), ab52625 (1/400 dilution); each using a separate fluorescent tyramide signal amplification system.

      Sodium citrate antigen retrieval (pH6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

      Image acquisition was performed with TissueFAXS Spectra (TissueGnostics).

    • Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)This image is courtesy of TissueGnostics Asia Pacific Limited

      10-color fluorescence multiplex immunohistochemical analysis of human lung cancer tissue (formalin-fixed paraffin-embedded section).

      Merged staining of anti-FOXP3 (ab215206; Cyan; TG540N), anti-PD1 (ab52587; Violet; TG700N), anti-CD163 (ab182422; Red; TG650N), anti-HLA-DR (ab92511; Yellow; TG570N), anti-CD4 (ab133616; Orange; TG620N), anti-CD8 alpha (ab101500; Purple; TG540S), anti-CD20 (ab9475; Grey; TG660S), anti-CD68 (ab192847; Green; TG520N), anti-Cytokeratin 19 (ab52625; Light blue; TG440N). TG470SN (dark blue) was used as a nuclear counter stain.

      The section was incubated in nine rounds of staining; in the order of ab215206 (1/100 dilution), ab52587 (1/200 dilution), ab182422 (1/300 dilution), ab92511 (1/200 dilution), ab133616 (1/600 dilution), ab101500 (1/300 dilution), ab9475 (1/100 dilution), ab192847 (1/300 dilution), ab52625 (1/400 dilution); each using a separate fluorescent tyramide signal amplification system.

      Sodium citrate antigen retrieval (pH6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

      Image acquisition was performed with TissueFAXS Spectra (TissueGnostics).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      Immunohistochemical staining of paraffin-embedded human skin with purified ab52625 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      ab52625 staining KRT19 in MCF7 cells, with negative expression in SHSY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab52625 at 2 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown. This product also work with 4% formaldehyde (10 min) fixation under the same testing conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labelling Cytokeratin 19 (green) with purified ab52625 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by ab150120 Alexa Fluor® 594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

      For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (ab150077).

      Alexa Fluor® 488 (ab192643) and Alexa Fluor® 647 (ab192980) conjugated versions are available for this clone.

    • Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      All lanes : Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625) at 1/45000 dilution (purified)

      Lane 1 : HepG2 (liver hepatocellular carcinoma cell line) cell lysate
      Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 44 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      ab52625 staining Cytokeratin 19in the human cell line MCF-7 (human breast carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody. 

      Isoytype control: Rabbit monoclonal IgG (Black) 

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue). 

      Alexa Fluorr® 488 (ab192643) and Alexa Fluorr® 647 (ab192980) conjugated versions are available for this clone. 

       

       

    • Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Different batches of ab52625 were tested on HepG2 (Human hepatocellular carcinoma epithelial cell) lysate at 0.2 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 40 kDa.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      Unpurified ab52625 showing positive staining in Breast carcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      Immunofluorescent staining of MCF-7 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab52625 at a dilution of 1/200. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200 and the cells were counter stained with DAPI.

    • Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with unpurified ab52625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52625, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      Unpurified ab52625 showing positive staining in Endometrial carcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      Unpurified ab52625 showing negative staining in Glioma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

      Unpurified ab52625 showing positive staining in Gastric adenocarcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      OI-RD Scanning - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
      Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (150)

    Publishing research using ab52625? Please let us know so that we can cite the reference in this datasheet.

    ab52625 has been referenced in 150 publications.

    • Han X  et al. Basal microvilli define the metabolic capacity and lethal phenotype of pancreatic cancer. J Pathol 253:304-314 (2021). PubMed: 33159698
    • Chung WC  et al. Loss of Jag1 cooperates with oncogenic Kras to induce pancreatic cystic neoplasms. Life Sci Alliance 4:N/A (2021). PubMed: 33268505
    • Kasuga A  et al. Oncogenic KRAS-expressing organoids with biliary epithelial stem cell properties give rise to biliary tract cancer in mice. Cancer Sci 112:1822-1838 (2021). PubMed: 33068050
    • Burchill MA  et al. Oxidized Low-Density Lipoprotein Drives Dysfunction of the Liver Lymphatic System. Cell Mol Gastroenterol Hepatol 11:573-595 (2021). PubMed: 32961356
    • de Boer JF  et al. Cholangiopathy and Biliary Fibrosis in Cyp2c70-Deficient Mice Are Fully Reversed by Ursodeoxycholic Acid. Cell Mol Gastroenterol Hepatol 11:1045-1069 (2021). PubMed: 33309945
    View all Publications for this product

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution (S1699)
    Permeabilization
    Yes - Wash buffer, blocking solution and secondary diluent was 1X TBS with 0.1% Tween-20
    Specification
    Pancreas
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    10% neutral buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 15 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    Intestine
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Brett Bell

    Verified customer

    Submitted Dec 28 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Protein Block as blocking agent for 30 minute(s) · Concentration: 100%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 18 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Breast cancer cells)
    Permeabilization
    No
    Specification
    Breast cancer cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 02 2020

    IHC - Wholemount abreview for Anti-Cytokeratin 19 antibody [EP1580Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Cornea)
    Specification
    Cornea
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 16 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 19 antibody [EP1580Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Pancreas Tissue)
    Permeabilization
    Yes - Tween 20
    Specification
    Pancreas Tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dhruvit Sutaria

    Verified customer

    Submitted Jun 28 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 19 antibody [EP1580Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse salivary gland)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    Mouse salivary gland
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Isabelle Miletich

    Verified customer

    Submitted Mar 07 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 19 antibody [EP1580Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tongue)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    tongue
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 26 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 19 antibody [EP1580Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Human Cell (HCC1143 cell lines)
    Specification
    HCC1143 cell lines
    Permeabilization
    Yes - 0.3% Triton X-100 in TBS
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 25 2015

    Flow Cytometry abreview for Anti-Cytokeratin 19 antibody [EP1580Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Sample
    Human Cell (CK19 positive and negative cell lines)
    Specification
    CK19 positive and negative cell lines
    Gating Strategy
    live cells, doubled were excluded based on FSC - SSC profile
    Preparation
    Cell harvesting/tissue preparation method: Cells were harvested with trypsin and fixed with 2% PFA / PBS for 15 min on ice. Following cell fixation, PFA was removed, cells were washed with ice-cold PBS, permeabilised and blocked with 0.2% Triton X-100 / 5% FBS / PBS for 30 min on ice prior to staining
    Sample buffer: PBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 05 2015

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