Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)
Key features and details
- Mouse monoclonal [KRT19/800] to Cytokeratin 19
- Suitable for: IHC-P, WB, ICC
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 19 antibody [KRT19/800]
See all Cytokeratin 19 primary antibodies -
Description
Mouse monoclonal [KRT19/800] to Cytokeratin 19 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICCmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Cytokeratin 19.
Database link: P08727 -
Positive control
- IHC-P: Human ovarian carcinoma, endometrial carcinoma, cervical carcinoma, bladder carcinoma, and pancreas tissues; rat stomach, colon and lung tissues; ICC: MCF7 cells; WB: Human lung lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab220193 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
KRT19/800 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 44 kDa.
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ICC |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 44 kDa. |
ICC
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Present in hair follicles at all stages of development. -
Domain
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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Database links
- Entrez Gene: 3880 Human
- Entrez Gene: 360626 Rat
- Omim: 148020 Human
- SwissProt: P08727 Human
- SwissProt: Q63279 Rat
- Unigene: 654568 Human
- Unigene: 9359 Rat
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Alternative names
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat colon tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.
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Immunocytochemistry analysis of MeOH fixed MCF7 (human breast adenocarcinoma cell line) cells labeling CK19 using ab220193 followed by Goat anti-mouse IgG-CF488 (Cyan). The nuclear counterstain is Reddot (Red).
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Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193) + Human lung lysate
Predicted band size: 44 kDa -
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human pancreas tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat lung tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervical carcinoma tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human bladder carcinoma tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human endometrial carcinoma tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human ovarian carcinoma tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.
Datasheets and documents
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Datasheet download
References (1)
ab220193 has been referenced in 1 publication.
- Dobie R et al. Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. Cell Rep 29:1832-1847.e8 (2019). PubMed: 31722201