Anti-Cytomegalovirus pp65 antibody [3A12] - BSA and Azide free (ab6503)
Key features and details
- Mouse monoclonal [3A12] to Cytomegalovirus pp65 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human cytomegalovirus
- Isotype: IgG2a
Overview
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Product name
Anti-Cytomegalovirus pp65 antibody [3A12] - BSA and Azide free
See all Cytomegalovirus pp65 primary antibodies -
Description
Mouse monoclonal [3A12] to Cytomegalovirus pp65 - BSA and Azide free -
Host species
Mouse -
Specificity
Cytomegalovirus pp65 (Lower Matrix Protein). -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human cytomegalovirus -
Immunogen
Tissue, cells or virus corresponding to Cytomegalovirus pp65.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3A12 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/16000.
|
|
| ICC/IF |
1/3200.
|
| Notes |
|---|
|
WB
1/16000. |
|
ICC/IF
1/3200. |
Target
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Relevance
Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens. -
Database links
- Entrez Gene: 1487754 Human cytomegalovirus
- SwissProt: P06725 Human cytomegalovirus
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Alternative names
- 65 kDa matrix phosphoprotein antibody
- 65 kDa phosphoprotein antibody
- Tegument protein pp65 antibody
- Tegument protein UL83 antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab6503 has been referenced in 26 publications.
- Vezzani G et al. The Human Cytomegalovirus UL116 Glycoprotein Is a Chaperone to Control gH-Based Complexes Levels on Virions. Front Microbiol 12:630121 (2021). PubMed: 33889136
- Gonzalez-Perez AC et al. The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts. mBio 12:N/A (2021). PubMed: 33947766
- Liu M et al. Ferulic acid inhibits LPS-induced apoptosis in bovine mammary epithelial cells by regulating the NF-κB and Nrf2 signalling pathways to restore mitochondrial dynamics and ROS generation. Vet Res 52:104 (2021). PubMed: 34256834
- Le-Trilling VTK et al. The Human Cytomegalovirus pUL145 Isoforms Act as Viral DDB1-Cullin-Associated Factors to Instruct Host Protein Degradation to Impede Innate Immunity. Cell Rep 30:2248-2260.e5 (2020). PubMed: 32075763
- Weathers SP et al. Glioblastoma-mediated Immune Dysfunction Limits CMV-specific T Cells and Therapeutic Responses: Results from a Phase I/II Trial. Clin Cancer Res 26:3565-3577 (2020). PubMed: 32299815