Anti-Cytosolic Phospholipase A2 antibody (ab58375)
Key features and details
- Rabbit polyclonal to Cytosolic Phospholipase A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Cytosolic Phospholipase A2 antibody
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Rabbit polyclonal to Cytosolic Phospholipase A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cytosolic Phospholipase A2 aa 450-550. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P47712 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab58375 was affinity purified from rabbit antiserum affinity chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 85 kDa).
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 85 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
Target
-
Function
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
Tissue specificity
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
Domain
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
Post-translational
modificationsActivated by phosphorylation at both Ser-505 and Ser-727. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
-
Database links
- Entrez Gene: 151056 Human
- Entrez Gene: 5321 Human
- Omim: 600522 Human
- SwissProt: P47712 Human
- SwissProt: Q6P1J6 Human
- Unigene: 444933 Human
- Unigene: 497200 Human
-
Alternative names
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
see all
Images
-
Western blot - Anti-Cytosolic Phospholipase A2 antibody (ab58375)All lanes : Anti-Cytosolic Phospholipase A2 antibody (ab58375) at 1/500 dilution
Lane 1 : HeLa cell extracts treated
with TNF alpha (20ng/ml, 30mins)
Lane 2 : HeLa cell extracts treated
with TNF alpha (20ng/ml, 30mins) with immunising peptide
Predicted band size: 85 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody (ab58375)ab58375 at 1/50-1/100 dilution staining Cytosolic Phospholipase A2 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody (ab58375)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of Human spleen tissue, staining Phospholipase A2 with ab58375.
Tissue was fixed with formaldehyde and blocked with blocking solution for 10 minutes at 37°C; antigen retrieval was by heat mediation in a cell conditioner. Samples were incubated with primary antibody (undiluted) for 1 hour at 37°C. A biotinylated-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (20)
ab58375 has been referenced in 20 publications.
- Sun J et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224
- Xue Y et al. Gigantol ameliorates CCl4-induced liver injury via preventing activation of JNK/cPLA2/12-LOX inflammatory pathway. Sci Rep 10:22265 (2020). PubMed: 33335297
- Ding Y et al. Annexin A1 attenuates neuroinflammation through FPR2/p38/COX-2 pathway after intracerebral hemorrhage in male mice. J Neurosci Res 98:168-178 (2020). PubMed: 31157469
- Holopainen M et al. Polyunsaturated fatty acids modify the extracellular vesicle membranes and increase the production of proresolving lipid mediators of human mesenchymal stromal cells. Biochim Biophys Acta Mol Cell Biol Lipids 1864:1350-1362 (2019). PubMed: 31207356
- Sun R et al. Annexin10 promotes extrahepatic cholangiocarcinoma metastasis by facilitating EMT via PLA2G4A/PGE2/STAT3 pathway. EBioMedicine 47:142-155 (2019). PubMed: 31492557