Name: Anti-DAZL antibody [EPR21028]
Brand: RabMAb
SKU: ab215718
Rating: 4 (8 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-DAZL antibody [EPR21028] (ab215718)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21028] to DAZL
Suitable for: ICC/IF, WB, IHC-P, IHC-Fr, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-DAZL antibody [EPR21028]
See all DAZL primary antibodies
Description

Rabbit monoclonal [EPR21028] to DAZL
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-P, IHC-Fr, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Horse, Cow, Pig
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat testis lysates. IHC-P: Human, mouse and rat testis tissues. IHC-Fr: Mouse and rat testis tissues. IP: Mouse testis lysate. ICC/IF: mES cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21028
Isotype

IgG
Research areas

Alternative Versions
- Anti-DAZL antibody [EPR21028] - BSA and Azide free (ab228135)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human DAZL protein (ab112279)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab215718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF	(2)	Use a concentration of 1 µg/ml.
WB	(1)	1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
IHC-P	(3)	1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Human); 1/4000 (Mouse and rat).
IHC-Fr	(1)	1/300. (Mouse); 1/60 (Rat). Perform heat mediated antigen retrieval by using sodium citrate buffer (pH 6.0).
IP		1/30.

Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Human); 1/4000 (Mouse and rat).
IHC-Fr 1/300. (Mouse); 1/60 (Rat). Perform heat mediated antigen retrieval by using sodium citrate buffer (pH 6.0).
IP 1/30.

Function

RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
Tissue specificity

Testis specific.
Sequence similarities

Belongs to the RRM DAZ family.
Contains 1 DAZ-like domain.
Contains 1 RRM (RNA recognition motif) domain.
Domain

The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
Cellular localization

Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
Target information above from: UniProt accession Q92904 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 530116 Cow
- Entrez Gene: 1618 Human
- Entrez Gene: 13164 Mouse
- Entrez Gene: 680486 Rat
- Omim: 601486 Human
- SwissProt: Q92904 Human
- SwissProt: Q64368 Mouse
- Unigene: 131179 Human
- Unigene: 280641 Mouse
see all
Alternative names
- DAZ homolog antibody
- DAZ like autosomal antibody
- DAZ-like autosomal antibody
- DAZH antibody
- DAZL 1 antibody
- DAZL antibody
- DAZL_HUMAN antibody
- DAZL1 antibody
- DAZLA antibody
- Deleted in azoospermia like 1 antibody
- Deleted in azoospermia like antibody
- Deleted in azoospermia like autosomal antibody
- Deleted in azoospermia-like 1 antibody
- Deleted in azoospermia-like antibody
- Germline specific RNA binding protein antibody
- MGC26406 antibody
- Spermatogenesis gene on the Y like autosomal antibody
- SPGY like autosomal antibody
- SPGY-like-autosomal antibody
- SPGYLA antibody
- Tpx2 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-DAZL antibody [EPR21028] (ab215718)

ab215718 staining DAZL in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab215718 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody [EPR21028] (ab215718)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DAZL with ab215718 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in primary spermatocytes of human testis (PMID: 24746554) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-DAZL antibody [EPR21028] (ab215718)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen mouse testis tissue labeling DAZL with ab215718 at 1/300 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® staining in the pachytene spermatocytes and secondary spermatocytes, not present in Sertoli, Leydig or myoid cells (PMID: 24746554).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-DAZL antibody [EPR21028] (ab215718)This image is courtesy of an Abreview submitted by Dr. Ina Theofel

Immunocytochemistry analysis of mESCs cultured in 2i conditions labelling DAZL with ab215718 at 1/500 dilution. Cells were fixed with 4% formaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/4000 was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue).
See Abreview
Western blot - Anti-DAZL antibody [EPR21028] (ab215718)This image is courtesy of an anonymous collaborator abreview.

All lanes : Anti-DAZL antibody [EPR21028] (ab215718) at 1/1000 dilution

Lane 1 : Mouse testis tissue lysate
Lane 2 : DAZL knockout mouse testis tissue lysate

Lysates/proteins at 25 µg per lane.

Secondary
All lanes : HRP-conjugated horse anti-rabbit IgG at 1/10000 dilution

Developed using the ECL technique.

Performed under non-reducing conditions.

Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

10% gel.

Blocked with 5% milk for 1 hour at room temperature.

Incubated with the primary antibody for 12 hours at 4°C in TBST.
See Abreview
Western blot - Anti-DAZL antibody [EPR21028] (ab215718)

All lanes : Anti-DAZL antibody [EPR21028] (ab215718) at 1/1000 dilution

Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Human testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 33 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Immunoprecipitation - Anti-DAZL antibody [EPR21028] (ab215718)

DAZL was immunoprecipitated from 0.35 mg mouse testis lysate with ab215718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215718 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: Mouse testis lysate 10 μg (Input).

Lane 2: ab215718 IP in mouse testis lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215718 in mouse testis lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody [EPR21028] (ab215718)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling DAZL with ab215718 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in spermatogonia and primary spermatocytes of mouse testis (PMID: 24746554) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody [EPR21028] (ab215718)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling DAZL with ab215718 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in spermatogonia and primary spermatocytes of rat testis (PMID: 24746554) is observed. Counterstained with hematoxylin

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-DAZL antibody [EPR21028] (ab215718)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen rat testis tissue labeling DAZL with ab215718 at 1/60 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in the pachytene spermatocytes and secondary spermatocytes, not present in Sertoli, Leydig or myoid cells (PMID: 24746554).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-DAZL antibody [EPR21028] (ab215718)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab215718? Please let us know so that we can cite the reference in this datasheet.

ab215718 has been referenced in 5 publications.

Choi HJ et al. Single-cell RNA sequencing of mitotic-arrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens. J Anim Sci Biotechnol 13:64 (2022). IHC-P ; Chicken . PubMed: 35659766
Phan TP et al. Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells. Genes Dev N/A:N/A (2022). IHC-P ; Mouse . PubMed: 35772791
Shakeel M et al. Seasonal changes in the expression of molecular markers of stallion germ cells. J Equine Vet Sci N/A:104109 (2022). IHC-P ; Horse . PubMed: 36029943
Yang CR et al. The RNA-binding protein DAZL functions as repressor and activator of mRNA translation during oocyte maturation. Nat Commun 11:1399 (2020). WB ; Mouse . PubMed: 32170089
Huss DJ et al. Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration. Front Cell Dev Biol 7:35 (2019). PubMed: 30984757

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Chicken Cell (chicken 10-day embryo gonad, cryosection)

Permeabilization

Yes - 0.5% Triton-X100

Specification

chicken 10-day embryo gonad, cryosection

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 25 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (mESCs cultured in 2i conditions)

Specification

mESCs cultured in 2i conditions

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Ina Theofel

Verified customer

Submitted Sep 04 2020

Application

Flow Cytometry

Sample

Mouse Cell (mESC)

Permeabilization

Yes - 1X Permeabilization Buffer from thermo

Specification

mESC

Preparation

Cell harvesting/tissue preparation method: The kit used for the internal staining is: http://tools.thermofisher.com/content/sfs/manuals/staining-intracellular-antigens-for-flow-cytometry.pdf
Sample buffer: Foxp3 Fixation/Permeabilization

Fixation

kit from thermofisher

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Maud de Dieuleveult

Verified customer

Submitted Nov 21 2019

Application

Western blot

Sample

Mouse Tissue lysate - whole (mouse Testis)

Gel Running Conditions

Non-reduced Denaturing (10% gel)

Loading amount

25 µg

Specification

mouse Testis

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 08 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Testis)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Testis

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Aug 23 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Testis)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Testis

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Aug 23 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Testis)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Testis

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Aug 20 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Testis, adult)

Permeabilization

Yes - 0.1% Triton X-100 in PBS

Specification

Testis, adult

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Bryan Niedenberger

Verified customer

Submitted Jan 30 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (5)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-DAZL antibody [EPR21028]

Immunocytochemistry/ Immunofluorescence abreview for Anti-DAZL antibody [EPR21028]

Flow Cytometry abreview for Anti-DAZL antibody [EPR21028]

Western blot abreview for Anti-DAZL antibody [EPR21028]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DAZL antibody [EPR21028]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DAZL antibody [EPR21028]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DAZL antibody [EPR21028]

Immunohistochemistry (Frozen sections) abreview for Anti-DAZL antibody [EPR21028]