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    products/primary-antibodies/dcamkl1-antibody-epr6085-ab109029.pdf

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Neuroscience Neurology process Growth and Development Axonal Guidance Proteins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-DCAMKL1 antibody [EPR6085] (ab109029)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (9)

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Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
  • Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
  • Anti-DCAMKL1 antibody [EPR6085] (ab109029)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6085] to DCAMKL1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-DCAMKL1 antibody [EPR6085]
    See all DCAMKL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6085] to DCAMKL1
  • Host species

    Rabbit
  • Specificity

    The mouse recommendation is for WB use only. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal lung, NIH3T3, and SH-SY5Y lysates, Human brain tissue
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6085
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis

Associated products

  • Alternative Versions

    • Anti-DCAMKL1 antibody [EPR6085] - BSA and Azide free (ab187184)
    • Alexa Fluor® 488 Anti-DCAMKL1 antibody [EPR6085] (ab202754)
    • Alexa Fluor® 647 Anti-DCAMKL1 antibody [EPR6085] (ab202755)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant human DCAMKL1 protein (ab101777)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa).
IHC-P (4)
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
  • Tissue specificity

    In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 2 doublecortin domains.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession O15075 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9201 Human
    • Entrez Gene: 13175 Mouse
    • Entrez Gene: 83825 Rat
    • Omim: 604742 Human
    • SwissProt: O15075 Human
    • SwissProt: Q9JLM8 Mouse
    • SwissProt: O08875 Rat
    • Unigene: 507755 Human
    • Unigene: 393242 Mouse
    • Unigene: 472264 Mouse
    • Unigene: 155540 Rat
    • Unigene: 80575 Rat
    see all
  • Alternative names

    • Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
    • CL1 antibody
    • CLICK1 antibody
    • Cpg16 antibody
    • DCDC3A antibody
    • Dcl antibody
    • Dclk antibody
    • Dclk1 antibody
    • DCLK1_HUMAN antibody
    • Doublecortin domain-containing protein 3A antibody
    • Doublecortin-like and CAM kinase-like 1 antibody
    • Doublecortin-like kinase 1 antibody
    • KIAA0369 antibody
    • Serine/threonine-protein kinase DCAMKL1 antibody
    • Serine/threonine-protein kinase DCLK1 antibody
    see all

Images

  • Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
    Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Knockout HAP1 whole cell lysate (20 µg)
    Lane 3: DCLK1  (KO) whole cell lysate (20 µg)
    Lane 4: Empty whole cell lysate (20 µg)
    Lane 5: Hu brain whole cell lysate (20 µg)
    Lanes 1 - 5: Merged signal (red and green). Green - ab109029 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109029 was shown to specifically react with DCLK1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and knockout samples were subjected to SDS-PAGE. Ab109029 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.


  • Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
    Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
    All lanes : Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/1000 dilution

    Lane 1 : Human fetal lung lysate
    Lane 2 : NIH3T3 lysate
    Lane 3 : SH-SY5Y lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 82 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109029 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-DCAMKL1 antibody [EPR6085] (ab109029)
    Anti-DCAMKL1 antibody [EPR6085] (ab109029)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (9)

Publishing research using ab109029? Please let us know so that we can cite the reference in this datasheet.

ab109029 has been referenced in 9 publications.

  • Liu J  et al. Extracellular vesicles-encapsulated let-7i shed from bone mesenchymal stem cells suppress lung cancer via KDM3A/DCLK1/FXYD3 axis. J Cell Mol Med 25:1911-1926 (2021). PubMed: 33350586
  • Ali N  et al. Doublecortin-like kinase 1 promotes hepatocyte clonogenicity and oncogenic programming via non-canonical ß-catenin-dependent mechanism. Sci Rep 10:10578 (2020). PubMed: 32601309
  • Lapiere A  et al. Prophylactic Faecalibacterium prausnitzii treatment prevents the acute breakdown of colonic epithelial barrier in a preclinical model of pelvic radiation disease. Gut Microbes 12:1-15 (2020). PubMed: 32985332
  • Fan M  et al. Divergent expression of DCLK1 in gastrointestinal neuroendocrine tumors and primary hepatic, gallbladder, and pancreatic neuroendocrine tumors. Int J Clin Exp Pathol 13:2249-2258 (2020). PubMed: 33042329
  • Osman J  et al. The WNT5A Agonist Foxy5 Reduces the Number of Colonic Cancer Stem Cells in a Xenograft Mouse Model of Human Colonic Cancer. Anticancer Res 39:1719-1728 (2019). PubMed: 30952711
  • Roy BC  et al. Co-localization of autophagy-related protein p62 with cancer stem cell marker dclk1 may hamper dclk1's elimination during colon cancer development and progression. Oncotarget 10:2340-2354 (2019). PubMed: 31040926
  • Pinzon-Guzman C  et al. Evaluation of Lineage Changes in the Gastric Mucosa Following Infection With Helicobacter pylori and Specified Intestinal Flora in INS-GAS Mice. J Histochem Cytochem N/A:22155418785621 (2018). PubMed: 29969055
  • Fan M  et al. Expression and prognostic significance of doublecortin-like kinase 1 in patients with hepatocellular carcinoma. Oncol Lett 14:7529-7537 (2017). PubMed: 29344199
  • Feng Y  et al. Sox9 induction, ectopic Paneth cells, and mitotic spindle axis defects in mouse colon adenomatous epithelium arising from conditional biallelic Apc inactivation. Am J Pathol 183:493-503 (2013). ICC/IF ; Mouse . PubMed: 23769888

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-DCAMKL1 antibody [EPR6085]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. Narin Ilgım Ardıç

Verified customer

Submitted May 23 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DCAMKL1 antibody [EPR6085]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 17 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DCAMKL1 antibody [EPR6085]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 17 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DCAMKL1 antibody [EPR6085]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 17 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DCAMKL1 antibody [EPR6085]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% Normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 10 2018

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