Recombinant Anti-DCAMKL1 antibody [EPR6085] (ab109029)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-505714-anti-dcamkl1-antibody-epr6085-immunohistochemistry-colon-rat.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6085] to DCAMKL1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-DCAMKL1 antibody [EPR6085]
See all DCAMKL1 primary antibodies -
Description
Rabbit monoclonal [EPR6085] to DCAMKL1 -
Host species
Rabbit -
Specificity
The human recommendation is based on the WB results. We do not guarantee IHC-P for human.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate, Mouse and rat cerebllum lysate, brain lysate and colon lysate, NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, HAP1 whole cell lysate, human brain lysate. IHC-P: Mouse stomach, Rat colon.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6085 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa).
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| IHC-P | (4) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The human recommendation is based on the WB results. We do not guarantee IHC-P for human. |
| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The human recommendation is based on the WB results. We do not guarantee IHC-P for human. |
Target
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Function
Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system. -
Tissue specificity
In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 2 doublecortin domains.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 9201 Human
- Entrez Gene: 13175 Mouse
- Entrez Gene: 83825 Rat
- Omim: 604742 Human
- SwissProt: O15075 Human
- SwissProt: Q9JLM8 Mouse
- SwissProt: O08875 Rat
- Unigene: 507755 Human
see all -
Alternative names
- Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
- CL1 antibody
- CLICK1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
Immunohistochemical analysis of Paraffin-embedded sections rat colon tissue labelling DCAMKL1 with ab109029 at 1/4000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat colon tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0)
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-505713-anti-dcamkl1-antibody-epr6085-immunohistochemistry-stomach-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)Immunohistochemical analysis of Paraffin-embedded sections mouse stomach tissue labelling DCAMKL1 with ab109029 at 1/4000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse stomach tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0)
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-505712-anti-dcamkl1-antibody-epr6085-westernblot-shsy5y-human.jpg)
Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/5000 dilution + SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 47,82 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The antibody recognizes isoforms of AL, AS, BS and BL.
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![Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-505711-anti-dcamkl1-antibody-epr6085-westernblot-mouse-rat.jpg)
Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)All lanes : Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse cerebllum lysate
Lane 4 : Rat cerebllum lysate
Lane 5 : Mouse colon lysate
Lane 6 : Rat colon lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 47,82 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
This antibody recognizes isoforms of AL, AS, BS and BL.
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![Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-276544-anti-dcamkl1-antibody-epr6085-western-blot.jpg)
Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Knockout HAP1 whole cell lysate (20 µg)
Lane 3: DCLK1 (KO) whole cell lysate (20 µg)
Lane 4: Empty whole cell lysate (20 µg)
Lane 5: Hu brain whole cell lysate (20 µg)
Lanes 1 - 5: Merged signal (red and green). Green - ab109029 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109029 was shown to specifically react with DCLK1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and knockout samples were subjected to SDS-PAGE. Ab109029 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-4-benefits-of-recombinant-antibodies.png)
Anti-DCAMKL1 antibody [EPR6085] (ab109029)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab109029 has been referenced in 15 publications.
- Liu J et al. Extracellular vesicles-encapsulated let-7i shed from bone mesenchymal stem cells suppress lung cancer via KDM3A/DCLK1/FXYD3 axis. J Cell Mol Med 25:1911-1926 (2021). PubMed: 33350586
- Kaji I et al. Cell differentiation is disrupted by MYO5B loss through Wnt/Notch imbalance. JCI Insight 6:N/A (2021). PubMed: 34197342
- Karoglu-Eravsar ET et al. Short-term dietary restriction maintains synaptic plasticity whereas short-term overfeeding alters cellular dynamics in the aged brain: evidence from the zebrafish model organism. Neurobiol Aging 106:169-182 (2021). PubMed: 34284260
- Ding L et al. Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity. Cancers (Basel) 13:N/A (2021). PubMed: 34830884
- Wang SP et al. FDI-6 inhibits the expression and function of FOXM1 to sensitize BRCA-proficient triple-negative breast cancer cells to Olaparib by regulating cell cycle progression and DNA damage repair. Cell Death Dis 12:1138 (2021). PubMed: 34880209