Recombinant Anti-DCAMKL1 antibody [EPR6085] (ab109029)
![Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-276544-anti-dcamkl1-antibody-epr6085-western-blot.jpg "Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6085] to DCAMKL1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-DCAMKL1 antibody [EPR6085]
See all DCAMKL1 primary antibodies -
Description
Rabbit monoclonal [EPR6085] to DCAMKL1 -
Host species
Rabbit -
Specificity
The mouse recommendation is for WB use only. We do not guarantee IHC-P for mouse and rat.
-
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human fetal lung, NIH3T3, and SH-SY5Y lysates, Human brain tissue
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6085 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa).
|
| IHC-P | (4) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system. -
Tissue specificity
In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 2 doublecortin domains.
Contains 1 protein kinase domain. - Information by UniProt
-
Database links
- Entrez Gene: 9201 Human
- Entrez Gene: 13175 Mouse
- Entrez Gene: 83825 Rat
- Omim: 604742 Human
- SwissProt: O15075 Human
- SwissProt: Q9JLM8 Mouse
- SwissProt: O08875 Rat
- Unigene: 507755 Human
see all -
Alternative names
- Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
- CL1 antibody
- CLICK1 antibody
see all
Images
-
Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Knockout HAP1 whole cell lysate (20 µg)
Lane 3: DCLK1 (KO) whole cell lysate (20 µg)
Lane 4: Empty whole cell lysate (20 µg)
Lane 5: Hu brain whole cell lysate (20 µg)
Lanes 1 - 5: Merged signal (red and green). Green - ab109029 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109029 was shown to specifically react with DCLK1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and knockout samples were subjected to SDS-PAGE. Ab109029 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-121620-anti-dcamkl1-antibody-epr6085-western-blot.jpg)
Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)All lanes : Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : NIH3T3 lysate
Lane 3 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 82 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-121619-anti-dcamkl1-antibody-epr6085-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109029 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-DCAMKL1 antibody [EPR6085] (ab109029)](https://www.abcam.com/ps/products/109/ab109029/Images/ab109029-4-benefits-of-recombinant-antibodies.png)
Anti-DCAMKL1 antibody [EPR6085] (ab109029)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab109029 has been referenced in 9 publications.
- Liu J et al. Extracellular vesicles-encapsulated let-7i shed from bone mesenchymal stem cells suppress lung cancer via KDM3A/DCLK1/FXYD3 axis. J Cell Mol Med 25:1911-1926 (2021). PubMed: 33350586
- Ali N et al. Doublecortin-like kinase 1 promotes hepatocyte clonogenicity and oncogenic programming via non-canonical ß-catenin-dependent mechanism. Sci Rep 10:10578 (2020). PubMed: 32601309
- Lapiere A et al. Prophylactic Faecalibacterium prausnitzii treatment prevents the acute breakdown of colonic epithelial barrier in a preclinical model of pelvic radiation disease. Gut Microbes 12:1-15 (2020). PubMed: 32985332
- Fan M et al. Divergent expression of DCLK1 in gastrointestinal neuroendocrine tumors and primary hepatic, gallbladder, and pancreatic neuroendocrine tumors. Int J Clin Exp Pathol 13:2249-2258 (2020). PubMed: 33042329
- Osman J et al. The WNT5A Agonist Foxy5 Reduces the Number of Colonic Cancer Stem Cells in a Xenograft Mouse Model of Human Colonic Cancer. Anticancer Res 39:1719-1728 (2019). PubMed: 30952711
- Roy BC et al. Co-localization of autophagy-related protein p62 with cancer stem cell marker dclk1 may hamper dclk1's elimination during colon cancer development and progression. Oncotarget 10:2340-2354 (2019). PubMed: 31040926
- Pinzon-Guzman C et al. Evaluation of Lineage Changes in the Gastric Mucosa Following Infection With Helicobacter pylori and Specified Intestinal Flora in INS-GAS Mice. J Histochem Cytochem N/A:22155418785621 (2018). PubMed: 29969055
- Fan M et al. Expression and prognostic significance of doublecortin-like kinase 1 in patients with hepatocellular carcinoma. Oncol Lett 14:7529-7537 (2017). PubMed: 29344199
- Feng Y et al. Sox9 induction, ectopic Paneth cells, and mitotic spindle axis defects in mouse colon adenomatous epithelium arising from conditional biallelic Apc inactivation. Am J Pathol 183:493-503 (2013). ICC/IF ; Mouse . PubMed: 23769888