Recombinant Anti-Dcp1a antibody [EPR13822] (ab183709)
![Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)](https://www.abcam.com/ps/products/183/ab183709/Images/ab183709-353559-antidcp1aantibodyepr13822westernblotknockouthek293dcp1ahepg2humanlysates183709gdb.jpg "Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13822] to Dcp1a
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Dcp1a antibody [EPR13822]
See all Dcp1a primary antibodies -
Description
Rabbit monoclonal [EPR13822] to Dcp1a -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK- 293, HeLa, and HepG2 whole cell lysate; IHC-P: Human kidney tissue; ICC/IF: HepG2 and HeLa cells; Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13822 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).
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| ICC/IF |
1/250.
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| Notes |
|---|
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Flow Cyt (Intra)
1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa). |
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ICC/IF
1/250. |
Target
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Function
Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. -
Tissue specificity
Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas. -
Sequence similarities
Belongs to the DCP1 family. -
Cellular localization
Cytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4. - Information by UniProt
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Database links
- Entrez Gene: 55802 Human
- Entrez Gene: 75901 Mouse
- Entrez Gene: 361109 Rat
- Omim: 607010 Human
- SwissProt: Q9NPI6 Human
- SwissProt: Q91YD3 Mouse
- Unigene: 476353 Human
- Unigene: 28733 Mouse
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Alternative names
- DCP1 decapping enzyme homolog A antibody
- Dcp1a antibody
- DCP1A_HUMAN antibody
see all
Images
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Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)All lanes : Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : DCP1A knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab183709 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab183709 was shown to specifically react with DCP1A in wild-type HEK-293 cells as signal was lost in DCP1A knockout cells. Wild-type and DCP1A knockout samples were subjected to SDS-PAGE. Ab183709 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)](https://www.abcam.com/ps/products/183/ab183709/Images/ab183709-215722-anti-dcp1a-antibody-epr13822-western-blot.jpg)
Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/10000 dilution + 293T cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)](https://www.abcam.com/ps/products/183/ab183709/Images/ab183709-215713-anti-dcp1a-antibody-epr13822-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dcp1a with ab183709 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp1a antibody [EPR13822] (ab183709)](https://www.abcam.com/ps/products/183/ab183709/Images/ab183709-215718-anti-dcp1a-antibody-epr13822-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp1a antibody [EPR13822] (ab183709)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Dcp1a with ab183709 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)](https://www.abcam.com/ps/products/183/ab183709/Images/ab183709-261897-anti-dcp1a-antibody-epr13822-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)ab183709 staining Dcp1a in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only
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![Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)](https://www.abcam.com/ps/products/183/ab183709/Images/ab183709-215720-anti-dcp1a-antibody-epr13822-western-blot.jpg)
Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)All lanes : Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Dcp1a antibody [EPR13822] (ab183709)](https://www.abcam.com/ps/products/183/ab183709/Images/ab183709-215709-anti-dcp1a-antibody-epr13822-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Dcp1a antibody [EPR13822] (ab183709)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Dcp1a with ab183709 at 1/150 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Anti-Dcp1a antibody [EPR13822] (ab183709)](https://www.abcam.com/ps/products/183/ab183709/Images/ab183709-8-benefits-of-recombinant-antibodies.png)
Anti-Dcp1a antibody [EPR13822] (ab183709)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab183709 has been referenced in 5 publications.
- Lai HC et al. Regulation of RNA degradation pathways during the lipopolysaccharide response in Macrophages. J Leukoc Biol 109:593-603 (2021). PubMed: 32829531
- Fan S et al. Enterovirus 71 2A Protease Inhibits P-Body Formation To Promote Viral RNA Synthesis. J Virol 95:e0092221 (2021). PubMed: 34287048
- Carter SD et al. Correlated cryogenic fluorescence microscopy and electron cryo-tomography shows that exogenous TRIM5a can form hexagonal lattices or autophagy aggregates in vivo. Proc Natl Acad Sci U S A 117:29702-29711 (2020). PubMed: 33154161
- Lessel D et al. Germline AGO2 mutations impair RNA interference and human neurological development. Nat Commun 11:5797 (2020). PubMed: 33199684
- Sharma NR et al. KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182. Nucleic Acids Res 47:9368-9385 (2019). PubMed: 31400113