Recombinant Anti-DcR1 antibody [EPR6162] (ab133658)
![Western blot - Anti-DcR1 antibody [EPR6162] (ab133658)](https://www.abcam.com/ps/products/133/ab133658/Images/ab133658-147612-anti-dcr1-antibody-epr6162-western-blot.jpg "Western blot - Anti-DcR1 antibody [EPR6162] (ab133658)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6162] to DcR1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DcR1 antibody [EPR6162]
See all DcR1 primary antibodies -
Description
Rabbit monoclonal [EPR6162] to DcR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human DcR1 aa 200-300. The exact sequence is proprietary.
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Positive control
- Human spleen, JAR, Human tonsil, Jurkat, A549 and Human fetal heart lysates; A549 cells.
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General notes
This antibody was developed as part of a collaboration with the lab of Shi-Yong Sun at Emory University.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6162 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 27, 75, 80 kDa (predicted molecular weight: 65 kDa).
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 27, 75, 80 kDa (predicted molecular weight: 65 kDa). |
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ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for IHC-P or IP.
Target
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Function
Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand. -
Tissue specificity
Higher expression in normal tissues than in tumor cell lines. Highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsN-glycosylated and O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8794 Human
- Omim: 603613 Human
- SwissProt: O14798 Human
- Unigene: 655801 Human
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Alternative names
- Antagonist decoy receptor for TRAIL/Apo 2L antibody
- Antagonist decoy receptor for TRAIL/Apo-2L antibody
- CD263 antibody
see all
Images
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Western blot - Anti-DcR1 antibody [EPR6162] (ab133658)All lanes : Anti-DcR1 antibody [EPR6162] (ab133658) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : JAR cell lysate
Lane 3 : Human tonsil lysate
Lane 4 : Jurkat cell lysate
Lane 5 : A549 cell lysate
Lane 6 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-DcR1 antibody [EPR6162] (ab133658)](https://www.abcam.com/ps/products/133/ab133658/Images/ab133658-147611-anti-dcr1-antibody-epr6162-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DcR1 antibody [EPR6162] (ab133658)Immunofluorescent analysis of A549 cells labelling DcR1 with ab133658 at 1/100 dilution. -
![Anti-DcR1 antibody [EPR6162] (ab133658)](https://www.abcam.com/ps/products/133/ab133658/Images/ab133658-3-benefits-of-recombinant-antibodies.png)
Anti-DcR1 antibody [EPR6162] (ab133658)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab133658 has been referenced in 3 publications.
- Chen QZ et al. HTNV Sensitizes Host Toward TRAIL-Mediated Apoptosis-A Pivotal Anti-hantaviral Role of TRAIL. Front Immunol 11:1072 (2020). PubMed: 32636833
- Fang Y et al. HIF-1a Mediates TRAIL-Induced Neuronal Apoptosis via Regulating DcR1 Expression Following Traumatic Brain Injury. Front Cell Neurosci 14:192 (2020). PubMed: 32848609
- Willms A et al. Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells. PLoS One 14:e0214847 (2019). PubMed: 30947287