Anti-DCXR antibody [4G4AF5] (ab110283)
![Western blot - Anti-DCXR antibody [4G4AF5] (ab110283)](https://www.abcam.com/ps/products/110/ab110283/Images/ab110283-123494-anti-dcxr-antibody-4g4af5-western-blot.gif "Western blot - Anti-DCXR antibody [4G4AF5] (ab110283)")
Key features and details
- Mouse monoclonal [4G4AF5] to DCXR
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-DCXR antibody [4G4AF5]
See all DCXR primary antibodies -
Description
Mouse monoclonal [4G4AF5] to DCXR -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Isolated human liver mitochondria; Human HDFn cells; HL-60 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. Purity near homogeneity as judged by SDS-PAGE. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
4G4AF5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
|
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| ICC/IF |
Use a concentration of 4 µg/ml.
|
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. |
|
ICC/IF
Use a concentration of 4 µg/ml. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules. -
Tissue specificity
Highly expressed in kidney, liver and epididymis. In the epididymis, it is mainly expressed in the proximal and distal sections of the corpus region. Weakly or not expressed in brain, lung, heart, spleen and testis. -
Involvement in disease
Note=The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Membrane. Probably recruited to membranes via an interaction with phosphatidylinositol. - Information by UniProt
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Database links
- Entrez Gene: 51181 Human
- Omim: 608347 Human
- SwissProt: Q7Z4W1 Human
- Unigene: 9857 Human
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Alternative names
- Carbonyl reductase 2 antibody
- Carbonyl reductase II antibody
- DCR antibody
see all
Images
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Western blot - Anti-DCXR antibody [4G4AF5] (ab110283)Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/ml + Human liver mitochondria at 20 µg
Secondary
Goat anti-mouse conjugated to AP
Predicted band size: 26 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-DCXR antibody [4G4AF5] (ab110283)](https://www.abcam.com/ps/products/110/ab110283/Images/ab110283-123493-anti-dcxr-antibody-4g4af5-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-DCXR antibody [4G4AF5] (ab110283)Immunocytochemistry image of ab110283 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110283 at 4 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane. -
![Flow Cytometry - Anti-DCXR antibody [4G4AF5] (ab110283)](https://www.abcam.com/ps/products/110/ab110283/Images/ab110283-123492-anti-dcxr-antibody-4g4af5-flow-cytometry.jpg)
Flow Cytometry - Anti-DCXR antibody [4G4AF5] (ab110283)HL-60 cells were stained with 1 µg/mL ab110283 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab110283 has been referenced in 2 publications.
- Murdica V et al. In vitro cultured human endometrial cells release extracellular vesicles that can be uptaken by spermatozoa. Sci Rep 10:8856 (2020). PubMed: 32483153
- Murdica V et al. Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation. Fertil Steril 111:897-908.e2 (2019). PubMed: 31029245