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    products/primary-antibodies/ddddk-tag-binds-to-flag-tag-sequence-antibody-ab95045.pdf

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Tags & Cell Markers Epitope Tags DDDDK Tag
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Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab95045)

  • Datasheet
  • SDS
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Key features and details

  • Goat polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: ELISA, WB, ICC, IHC-P
  • Reacts with: Species independent
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
  • Description

    Goat polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, WB, ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.

  • General notes

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • DDDDK Tag

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab95045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use a concentration of 0.03 - 10 µg/ml. Coating: 2-10 ug/ml; Primary: 0.03-1 ug/ml
WB
Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 1 kDa. Colorimetric: 0.1-1 ug/ml; Chemiluminescent: 0.03-1 ug/ml
ICC
Use a concentration of 0.5 - 5 µg/ml.
IHC-P
Use at an assay dependent dilution.
Notes
ELISA
Use a concentration of 0.03 - 10 µg/ml. Coating: 2-10 ug/ml; Primary: 0.03-1 ug/ml
WB
Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 1 kDa. Colorimetric: 0.1-1 ug/ml; Chemiluminescent: 0.03-1 ug/ml
ICC
Use a concentration of 0.5 - 5 µg/ml.
IHC-P
Use at an assay dependent dilution.

Target

  • Relevance

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
  • Alternative names

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK (also known as the FLAG® tag sequence) antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab95045? Please let us know so that we can cite the reference in this datasheet.

ab95045 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293 EBV/WT)
Specification
HEK293 EBV/WT
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Danusia Vanoaica

Verified customer

Submitted Jan 18 2022

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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