Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab95045)
Key features and details
- Goat polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: ELISA, WB, ICC, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
Goat polyclonal to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Goat -
Tested applications
Suitable for: ELISA, WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
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General notes
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 0.03 - 10 µg/ml. Coating: 2-10 ug/ml; Primary: 0.03-1 ug/ml
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| WB |
Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 1 kDa. Colorimetric: 0.1-1 ug/ml; Chemiluminescent: 0.03-1 ug/ml
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| ICC |
Use a concentration of 0.5 - 5 µg/ml.
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| IHC-P |
Use at an assay dependent dilution.
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| Notes |
|---|
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ELISA
Use a concentration of 0.03 - 10 µg/ml. Coating: 2-10 ug/ml; Primary: 0.03-1 ug/ml |
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WB
Use a concentration of 0.03 - 1 µg/ml. Predicted molecular weight: 1 kDa. Colorimetric: 0.1-1 ug/ml; Chemiluminescent: 0.03-1 ug/ml |
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ICC
Use a concentration of 0.5 - 5 µg/ml. |
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IHC-P
Use at an assay dependent dilution. |
Target
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Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab95045 has been referenced in 2 publications.
- Pagano R et al. Arc controls alcohol cue relapse by a central amygdala mechanism. Mol Psychiatry 28:733-745 (2023). PubMed: 36357670
- Zhang Z et al. The Zinc-Finger protein ZCCHC3 inhibits LINE-1 retrotransposition. Front Microbiol 13:891852 (2022). PubMed: 36274734