Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)
![Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)](https://www.abcam.com/ps/products/125/ab125243/Images/ab125243-139413-anti-ddddk-tag-antibody-fg4r-equivalent-to-flag-antibodies-from-sigma-western-blot.jpg "Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)")
Key features and details
- Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB, ELISA, Dot blot, IP, In situ hybridization
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
Overview
-
Product name
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Mouse -
Specificity
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged (also known as the FLAG® tag sequence) fusion proteins.
-
Tested applications
Suitable for: WB, ELISA, Dot blot, IP, In situ hybridizationmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
Sequence:DYKDDDDK
-
Positive control
- HEK293 cells transfected with DYKDDDDK (also known as the FLAG® tag sequence).
-
General notes
If stored at 4°C please add 0.1% sodium azide.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab125243 was purified by protein A affinity chromatography from TCS -
Clonality
Monoclonal -
Clone number
FG4R -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/3000.
(with ECL) |
|
| ELISA |
Use at an assay dependent concentration.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| In situ hybridization |
1/500 - 1/2000.
|
| Notes |
|---|
|
WB
1/1000 - 1/3000. (with ECL) |
|
ELISA
Use at an assay dependent concentration. |
|
Dot blot
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
In situ hybridization
1/500 - 1/2000. |
Target
-
Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Images
-
Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)All lanes : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) at 1/1000 dilution
Lane 1 : HEK293 cell lysate non-transfected
Lane 2 : HEK293 cell lysate transfected with DDDDK tagged protein vector
Developed using the ECL technique.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (17)
ab125243 has been referenced in 17 publications.
- Su H et al. Grass Carp Reovirus Major Outer Capsid Protein VP4 Interacts with RNA Sensor RIG-I to Suppress Interferon Response. Biomolecules 10:N/A (2020). PubMed: 32268551
- Chen SH & Goller CC Harnessing single-stranded DNA binding protein to explore protein-protein and protein-DNA interactions. Biochem Mol Biol Educ 48:181-190 (2020). PubMed: 31850676
- Li JY et al. The zebrafish NLRP3 inflammasome has functional roles in ASC-dependent interleukin-1ß maturation and gasdermin E-mediated pyroptosis. J Biol Chem 295:1120-1141 (2020). PubMed: 31852739
- Ma L et al. The m6A reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function. Redox Biol 38:101801 (2020). PubMed: 33232910
- Min JS et al. A Cell-Based Reporter Assay for Screening Inhibitors of MERS Coronavirus RNA-Dependent RNA Polymerase Activity. J Clin Med 9:N/A (2020). PubMed: 32727069