Recombinant Anti-DDR2 antibody [EPR18826-119] (ab280354)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18826-119] to DDR2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-DDR2 antibody [EPR18826-119]
See all DDR2 primary antibodies -
Description
Rabbit monoclonal [EPR18826-119] to DDR2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HT-1080, HUVEC, A-204, A375, Human stomach, ovary, lung, Mouse ovary, lung, C2C12, C6, Rat ovary and His-tagged human DDR2 recombinant protein (aa22-399).
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18826-119 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 97 kDa.
|
Notes |
---|
WB
1/1000. Predicted molecular weight: 97 kDa. |
Target
-
Function
This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. -
Tissue specificity
The major 10 kDa transcript is expressed in high levels in heart and lung, less in brain, placenta, liver, skeletal muscle, pancreas, and kidney. -
Involvement in disease
Defects in DDR2 are the cause of spondyloepimetaphyseal dysplasia short limb-hand type (SEMD-SL) [MIM:271665]. A bone disease characterized by short-limbed dwarfism, a narrow chest with pectus excavatum, brachydactyly in the hands and feet, a characteristic craniofacial appearance and premature calcifications. The radiological findings are distinctive and comprise short long bones throughout the skeleton with striking epiphyses that are stippled, flattened and fragmented and flared, irregular metaphyses. Platyspondyly in the spine with wide intervertebral spaces is observed and some vertebral bodies are pear-shaped with central humps, anterior protrusions and posterior scalloping. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 F5/8 type C domain.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4921 Human
- Entrez Gene: 18214 Mouse
- Entrez Gene: 685781 Rat
- Omim: 191311 Human
- SwissProt: Q16832 Human
- SwissProt: Q62371 Mouse
- Unigene: 275757 Human
- Unigene: 593833 Human
see all -
Alternative names
- CD167 antigen-like family member B antibody
- CD167b antibody
- CD167b antigen antibody
see all
Images
-
All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 3 : A-204 (human muscle rhabdomyosarcoma) whole cell lysate
Lane 4 : A375 (human malignant melanoma epithelial cell) whole cell lysate
Lane 5 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).
Negative control: MDA-MB-231 (PMID: 28147276, 29616590)
Exposure time: 158 seconds.
-
All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : Human stomach tissue lysate
Lane 2 : Human ovary tissue lysate
Lane 3 : Human lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).
Exposure time: 3 minutes.
-
All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : Mouse ovary tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).
Exposure time: 3 minutes.
-
All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : Rat ovary tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).
Exposure time: 3 minutes.
-
All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : His-tagged human DDR2 recombinant protein (aa22-399)
Lane 2 : His-tagged human DDR1 recombinant protein (aa21-416)
Lysates/proteins at 0.03 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody can not recognize human DDR1.
Both recombinant proteins are made in house and extracted from HEK-293 expression systems.
Exposure time: 8 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab280354 has not yet been referenced specifically in any publications.