Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)
![Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)](https://www.abcam.com/ps/products/27/ab27591/Images/ab27591-35-anti-DDX4-MVH-antibody-mAbcam27591-immunocytochemistry-immunofluorescence-mES-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)")
Key features and details
- Mouse monoclonal [mAbcam27591] to DDX4 / MVH
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human, Common marmoset
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-DDX4 / MVH antibody [mAbcam27591]
See all DDX4 / MVH primary antibodies -
Description
Mouse monoclonal [mAbcam27591] to DDX4 / MVH -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Common marmoset
Predicted to work with: Rabbit, Pig
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Immunogen
Synthetic peptide corresponding to Human DDX4/ MVH aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: Mouse testis tissue lysate and mouse EG whole cell lysate. IHC-P: Human normal testis tissue and adult marmoset testis tissue. ICC/IF: mES cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam27591 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 - 10 µg/ml.
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| IHC-P | (3) |
Use a concentration of 0.1 - 1 µg/ml.
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| IHC-Fr | (4) |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 76 kDa).
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| Notes |
|---|
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
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IHC-P
Use a concentration of 0.1 - 1 µg/ml. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 76 kDa). |
Target
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Function
Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. -
Tissue specificity
Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. - Information by UniProt
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Database links
- Entrez Gene: 54514 Human
- Entrez Gene: 13206 Mouse
- Omim: 605281 Human
- SwissProt: Q9NQI0 Human
- SwissProt: Q61496 Mouse
- SwissProt: Q6GWX0 Pig
- Unigene: 223581 Human
- Unigene: 12818 Mouse
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Alternative names
- DDX 4 antibody
- Ddx4 antibody
- DDX4_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)ab27591 staining DDX4/MVH in mES cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab27591 at 10μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
![Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)](https://www.abcam.com/ps/products/27/ab27591/Images/ab27591-34-anti-ddx4-mvh-mabcam27591-antibody-immunocytochemistry-mes-embryonic-stem-cell-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)ab27591 staining DDX4/MVH in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab27591 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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![Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)](https://www.abcam.com/ps/products/27/ab27591/Images/ab27591-263535-anti-ddx4-mvh-antibody-mabcam27591-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)This image is courtesy of an Abreview by Bryan Niedenberger.ab27591 staining DDX4 / MVH antibody in pig tissue sections (testis, postnatal day 42) by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% 0.1% Triton X-100 in PBS and blocked with 3% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. Ab150110l at a dilution of 1/500 was used as the secondary antibody.
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![Western blot - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)](https://www.abcam.com/ps/products/27/ab27591/Images/ab27591-38523-anti-ddx4-mvh-antibody-mabcam27591-western-blot.jpg)
Western blot - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)All lanes : Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591) at 5 µg/ml (Blocked with 3% Milk)
Lane 1 : Mouse Testis Tissue Lysate
Lane 2 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)](https://www.abcam.com/ps/products/27/ab27591/Images/ab27591-38520-anti-ddx4-mvh-antibody-mabcam27591-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)This image is courtesy of an abreview from Zachary Yu-Ching Lin.IHC-P image of DDX4 / MVH (ab27591) on adult marmoset testis. The sections were fixed with Formaldehyde and blocked in 5% milk for 30 minutes, before incubation with the DDX4 antibody (1/50 dilution) for 18 hours at 4°C. Cytoplasmic staining is seen with ab27591 (green), DAPI was used to stain nuclei (blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)](https://www.abcam.com/ps/products/27/ab27591/Images/ab27591-391445-antiddx4mvhantibodyimmunohistochemistrynormaltestishuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [mAbcam27591] (ab27591)IHC image of DDX4/MVH antibody staining in a section of formalin-fixed paraffin-embedded normal human testis* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab27561, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Datasheets and documents
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SDS download
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Datasheet download
References (61)
ab27591 has been referenced in 61 publications.
- Tanimoto R et al. Blocking estrogen-induced AMH expression is crucial for normal follicle formation. Development 148:N/A (2021). PubMed: 33658225
- Wang JJ et al. Single-cell transcriptome dissection of the toxic impact of Di (2-ethylhexyl) phthalate on primordial follicle assembly. Theranostics 11:4992-5009 (2021). PubMed: 33754040
- Wang M et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
- Habara O et al. WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility. Development 148:N/A (2021). PubMed: 33914868
- Frost ER et al. Two alternative methods for the retrieval of somatic cell populations from the mouse ovary. Mol Hum Reprod 27:N/A (2021). PubMed: 33973015