Anti-Deadpan antibody [11D1CH11] (ab195172)
![Immunohistochemistry - Anti-Deadpan antibody [11D1CH11] (ab195172)](https://www.abcam.com/ps/products/195/ab195172/Images/ab195172-232848-anti-deadpan-antibody-11d1ch11-immunohistochemistry.jpg "Immunohistochemistry - Anti-Deadpan antibody [11D1CH11] (ab195172)")
Key features and details
- Rat monoclonal [11D1CH11] to Deadpan
- Suitable for: IHC
- Reacts with: Drosophila melanogaster
- Isotype: IgG2b
Overview
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Product name
Anti-Deadpan antibody [11D1CH11]
See all Deadpan primary antibodies -
Description
Rat monoclonal [11D1CH11] to Deadpan -
Host species
Rat -
Tested applications
Suitable for: IHCmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Drosophila stage 11 embryos
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purification notes
>95% based on SDS-PAGE -
Clonality
Monoclonal -
Clone number
11D1CH11 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC
Use at an assay dependent concentration. |
Target
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Relevance
Transcriptional repressor of genes that require a bHLH protein for their transcription. Likely to be involved in the functional rather than the morphological differentiation of neurons. Loss of DPN function results in weak motor activity, lethargic behavior, and death. Implicated in sex determination as Groucho-DPN complex act directly to repress SXL transcription -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 35800 Drosophila melanogaster
- SwissProt: Q26263 Drosophila melanogaster
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Alternative names
- deadpan antibody
- Dpn antibody
- Protein deadpan antibody
Images
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Immunohistochemistry - Anti-Deadpan antibody [11D1CH11] (ab195172)
Immunohistochemistry analysis Drosophila stage 11 embryo labeling Deadpan with ab195172 at 10 µg/mL, followed by Alexa405 conjugated goat anti-rat secondary (at 10µg/mL).
Datasheets and documents
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Datasheet download
References (8)
ab195172 has been referenced in 8 publications.
- Martin E et al. Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment. Dev Cell 56:687-701.e7 (2021). PubMed: 33535069
- Link N et al. Mutations in ANKLE2, a ZIKA Virus Target, Disrupt an Asymmetric Cell Division Pathway in Drosophila Neuroblasts to Cause Microcephaly. Dev Cell 51:713-729.e6 (2019). PubMed: 31735666
- Neves A & Eisenman RN Distinct gene-selective roles for a network of core promoter factors in Drosophila neural stem cell identity. Biol Open 8:N/A (2019). PubMed: 30948355
- Chen D et al. Shep regulates Drosophila neuronal remodeling by controlling transcription of its chromatin targets. Development 145:N/A (2018). PubMed: 29158441
- Vissers JHA et al. The Scalloped and Nerfin-1 Transcription Factors Cooperate to Maintain Neuronal Cell Fate. Cell Rep 25:1561-1576.e7 (2018). PubMed: 30404010
- Shah PS et al. Comparative Flavivirus-Host Protein Interaction Mapping Reveals Mechanisms of Dengue and Zika Virus Pathogenesis. Cell 175:1931-1945.e18 (2018). PubMed: 30550790
- Yang CP et al. Imp and Syp RNA-binding proteins govern decommissioning ofDrosophilaneural stem cells. Development 144:3454-3464 (2017). PubMed: 28851709
- Schoborg T et al. An Asp-CaM complex is required for centrosome-pole cohesion and centrosome inheritance in neural stem cells. J Cell Biol 211:987-98 (2015). PubMed: 26620907
