Anti-DECR1 antibody [8B1AD10] (ab110287)
![Immunoprecipitation - Anti-DECR1 antibody [8B1AD10] (ab110287)](https://www.abcam.com/ps/products/110/ab110287/Images/ab110287-123509-anti-decr1-antibody-8b1ad10-immunoprecipitation.gif "Immunoprecipitation - Anti-DECR1 antibody [8B1AD10] (ab110287)")
Key features and details
- Mouse monoclonal [8B1AD10] to DECR1
- Suitable for: IP, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-DECR1 antibody [8B1AD10]
See all DECR1 primary antibodies -
Description
Mouse monoclonal [8B1AD10] to DECR1 -
Host species
Mouse -
Tested applications
Suitable for: IP, ICC/IF, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- MRC5 fibroblasts; Human cerebellum tissue; Human liver mitochondria; HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
8B1AD10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration. This antibody will only immunoprecipitate DECR1 from isolated mitochondria.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. This antibody will only immunoprecipitate DECR1 from isolated mitochondria. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. -
Tissue specificity
Heart = liver = pancreas > kidney >> skeletal muscle = lung. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 1666 Human
- Omim: 222745 Human
- SwissProt: Q16698 Human
- Unigene: 492212 Human
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Alternative names
- 1200012F07Rik antibody
- 2 antibody
- 2,4 dienoyl CoA reductase [NADPH] antibody
see all
Images
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Immunoprecipitation - Anti-DECR1 antibody [8B1AD10] (ab110287)DECR1 immunocaptured from human liver mitochondria using ab110287.
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![Flow Cytometry - Anti-DECR1 antibody [8B1AD10] (ab110287)](https://www.abcam.com/ps/products/110/ab110287/Images/ab110287-123508-anti-decr1-antibody-8b1ad10-flow-cytometry.jpg)
Flow Cytometry - Anti-DECR1 antibody [8B1AD10] (ab110287)HeLa cells were stained with 1 µg/mL ab110287 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DECR1 antibody [8B1AD10] (ab110287)](https://www.abcam.com/ps/products/110/ab110287/Images/ab110287-123507-anti-decr1-antibody-8b1ad10-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DECR1 antibody [8B1AD10] (ab110287)ab110287 staining DECR1 in formalin-fixed, paraffin-embedded Human cerebellum tissue by Immunohistochemistry. DECR1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. -
![Immunocytochemistry/ Immunofluorescence - Anti-DECR1 antibody [8B1AD10] (ab110287)](https://www.abcam.com/ps/products/110/ab110287/Images/ab110287-123506-anti-decr1-antibody-8b1ad10-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-DECR1 antibody [8B1AD10] (ab110287)MRC5 fibroblasts labeled with 1 µg/ml ab110287 and detected with goat anti-mouse secondary conjugated to FITC.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110287 has not yet been referenced specifically in any publications.