Anti-DeltaD antibody [Dld 5C7/2, zdd2] (ab73331)
Key features and details
- Mouse monoclonal [Dld 5C7/2, zdd2] to DeltaD
- Suitable for: WB, ELISA, ICC/IF, IHC-Fr, IHC - Wholemount, Flow Cyt
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-DeltaD antibody [Dld 5C7/2, zdd2] -
Description
Mouse monoclonal [Dld 5C7/2, zdd2] to DeltaD -
Host species
Mouse -
Specificity
This antibody recognises the extracellular domain of zebrafish DeltaD. This antibody does not detect DeltaC; but it cross-reacts weakly with DeltaA. -
Tested applications
Suitable for: WB, ELISA, ICC/IF, IHC-Fr, IHC - Wholemount, Flow Cytmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Extracellular DeltaD peptide (Zebrafish).
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Epitope
Zebrafish DeltaD protein extracellular domain. -
Positive control
- HEK293 cells transfected with deltaD or zebrafish embryo.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Dld 5C7/2, zdd2 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| IHC - Wholemount | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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WB
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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IHC - Wholemount
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
DeltaD is one of the 4 members of the zebrafish Delta family of Notch ligands, important in the control of cell differentiation in many tissues. DeltaD acts as a ligand for Notch receptors and is involved in primary neurogenesis and somitogenesis. It can activate Notch receptors, thereby playing a key role in lateral inhibition, a process that prevents the immediate neighbors of each nascent neural cell from simultaneously embarking on neural differentiation. It is required in somite segmentation to keep the oscillations of neighboring presomitic mesoderm cells synchronized. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 30138 Zebrafish
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Alternative names
- Aei antibody
- After eight antibody
- Dld antibody
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab73331 has been referenced in 11 publications.
- Zhao X et al. Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division. Sci Adv 7:N/A (2021). PubMed: 34117063
- Sehgal P et al. LncRNA VEAL2 regulates PRKCB2 to modulate endothelial permeability in diabetic retinopathy. EMBO J 40:e107134 (2021). PubMed: 34180064
- Sharma P et al. Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis. Sci Rep 9:9958 (2019). PubMed: 31292468
- Gallagher TL et al. Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation. Dev Biol 429:225-239 (2017). PubMed: 28648842
- Tong K et al. Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo. Methods Mol Biol N/A:N/A (2017). PubMed: 28434166
