Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)
Key features and details
- Mouse monoclonal [DN3] to Dengue Virus NS1 glycoprotein
- Suitable for: WB, ICC/IF
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4
- Isotype: IgG1
Overview
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Product name
Anti-Dengue Virus NS1 glycoprotein antibody [DN3]
See all Dengue Virus NS1 glycoprotein primary antibodies -
Description
Mouse monoclonal [DN3] to Dengue Virus NS1 glycoprotein -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4 -
Immunogen
Full length native protein purified from Dengue Virus 2 (16681) infected supernatant.
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Positive control
- It is important to ensure that DEN4 infection is effective. DEN4 is notoriously difficult to grow to good titres and NS1 antigens will not be found if infection is not well established.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 10% BSA, 89.9% RPMI 1640 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
DN3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 40, 80 kDa (predicted molecular weight: 40, 80 kDa).
Block membrane with PBS containing 5% non-fat skimmed milk (PBS-SM) for 30 minutes. Wash membrane and probe with serum diluted in PBS-SM with 10 mM sodium azide by incubating overnight on a rocker at room temperature. |
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ICC/IF |
1/5 - 1/20.
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Notes |
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WB
1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 40, 80 kDa (predicted molecular weight: 40, 80 kDa). Block membrane with PBS containing 5% non-fat skimmed milk (PBS-SM) for 30 minutes. Wash membrane and probe with serum diluted in PBS-SM with 10 mM sodium azide by incubating overnight on a rocker at room temperature. |
ICC/IF
1/5 - 1/20. |
Target
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Relevance
NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever. -
Database links
- Entrez Gene: 5075725 Dengue virus 1
- Entrez Gene: 5075727 Dengue virus 3
- SwissProt: P17763 Dengue virus 1
- SwissProt: P27909 Dengue virus 1
- SwissProt: P29990 Dengue virus 2
- SwissProt: Q6YMS4 Dengue virus 3
- SwissProt: P09866 Dengue virus 4
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Alternative names
- Dengue NS1 antibody
- Dengue Virus non structural protein 1 antibody
Images
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All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)
Lane 1 : Dengue Virus 1 infected C6/36 cell lysate (unheated)
Lane 2 : Dengue Virus 1 infected C6/36 cell lysate (boiled)
Lane 3 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (unheated)
Lane 4 : Dengue Virus 2 (NGC) infected C6/36 cell lysate (boiled)
Lane 5 : Dengue Virus 2 (16681) infected C6/36 cell lysate (unheated)
Lane 6 : Dengue Virus 2 (16681) infected C6/36 cell lysate (boiled)
Lane 7 : Dengue Virus 3 infected C6/36 cell lysate (unheated)
Lane 8 : Dengue Virus 3 infected C6/36 cell lysate (boiled)
Lane 9 : Dengue Virus 4 infected C6/36 cell lysate (unheated)
Lane 10 : Dengue Virus 4 infected C6/36 cell lysate (boiled)
Predicted band size: 40, 80 kDa
Observed band size: 40,80 kDa why is the actual band size different from the predicted?
Recognises NS1 dimer (~80 kDa) in unheated samples and NS1 monomer (~40 kDa) when samples are boiled. -
Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)Dengue Virus 2 infected Vero cells stained according to protocol with ab41349 antibody against Dengue Virus envelope glycoprotein NS1 (green). Cell nuclei are blue.
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Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus NS1 glycoprotein antibody [DN3] (ab41616)Image from Heaton NS et al, Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17345-50. Epub 2010 Sep 20, Fig 2. DOI 10.1073/pnas.1010811107ab41616 staining NS1 glycoprotein in Dengue Virus 2 infected Huh-7.5 cells by Immunocytochemistry/ Immunofluorescence.
Huh-7.5 cells were plated on glass coverslips, infected with Dengue Virus 2, fixed in methanol, and blocked, and antibody was added in 1× PBS + 0.1% saponin. Antibodies used include FASN polyclonal, left image (ab3844) or NS1 monoclonal antibody, middle image (ab41616). Secondary antibodies were species-appropriate Alexa-Fluor 488 or 594 secondary antibody. Stained coverslips were mounted with DAPI, right merged image.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab41616 has been referenced in 15 publications.
- Niu C et al. Differences in the Transmission of Dengue Fever by Different Serotypes of Dengue Virus. Vector Borne Zoonotic Dis 20:143-150 (2020). PubMed: 31503521
- Xie X et al. Dengue NS2A Protein Orchestrates Virus Assembly. Cell Host Microbe 26:606-622.e8 (2019). PubMed: 31631053
- Austin Suthanthiraraj PP & Sen AK Localized surface plasmon resonance (LSPR) biosensor based on thermally annealed silver nanostructures with on-chip blood-plasma separation for the detection of dengue non-structural protein NS1 antigen. Biosens Bioelectron 132:38-46 (2019). PubMed: 30851494
- Gomes GF et al. Differential Microglial Morphological Response, TNFa, and Viral Load in Sedentary-like and Active Murine Models After Systemic Non-neurotropic Dengue Virus Infection. J Histochem Cytochem 67:419-439 (2019). PubMed: 30924711
- Marques LÉC et al. Transient Expression of Dengue Virus NS1 Antigen in Nicotiana benthamiana for Use as a Diagnostic Antigen. Front Plant Sci 10:1674 (2019). PubMed: 32010161