Recombinant Anti-Desmocollin 1 antibody [EPR8220] (ab150382)
![Western blot - Anti-Desmocollin 1 antibody [EPR8220] (ab150382)](https://www.abcam.com/ps/products/150/ab150382/Images/ab150382-396921-anti-desmocollin-1-antibody-epr8220-westernblot-human-mouse-rat.jpg "Western blot - Anti-Desmocollin 1 antibody [EPR8220] (ab150382)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8220] to Desmocollin 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Desmocollin 1 antibody [EPR8220]
See all Desmocollin 1 primary antibodies -
Description
Rabbit monoclonal [EPR8220] to Desmocollin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Desmocollin 1 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q08554 -
Positive control
- WB: A375 and A431 whole cell lysates. Human skin tissue lysate. Mouse brain, skin, and kidney tissue lysates. Rat brain and kidney tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8220 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 100 kDa.
For unpurified use at 1/1000 - 1/10000. |
| Notes |
|---|
|
WB
1/10000. Predicted molecular weight: 100 kDa. For unpurified use at 1/1000 - 1/10000. |
Target
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Relevance
Desmocollin 1 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It may contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Desmocollin 1 is linked to the keratinization of epithelial tissues. -
Cellular localization
Cell membrane; Single-pass type I membrane protein. Cell junction; desmosome. -
Database links
- Entrez Gene: 1823 Human
- Entrez Gene: 13505 Mouse
- Entrez Gene: 291759 Rat
- Omim: 125643 Human
- SwissProt: Q08554 Human
- SwissProt: P55849 Mouse
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Alternative names
- Cadherin family member 1 antibody
- CDHF1 antibody
- Desmocollin1 antibody
see all
Images
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Western blot - Anti-Desmocollin 1 antibody [EPR8220] (ab150382)All lanes : Anti-Desmocollin 1 antibody [EPR8220] (ab150382) at 1/10000 dilution (purified)
Lane 1 : A375 (Human malignant melanoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : Human skin lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Mouse kidney lysate
Lane 7 : Rat brain lysate
Lane 8 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDaWe are unsure about the extra bands. They might be the deglycosylated forms as described in PMID: 25918164.
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![Western blot - Anti-Desmocollin 1 antibody [EPR8220] (ab150382)](https://www.abcam.com/ps/products/150/ab150382/Images/ab150382-482332-antidesmocollin-1-antibody-epr8220-western-blot-human-293t.jpg)
Western blot - Anti-Desmocollin 1 antibody [EPR8220] (ab150382)All lanes : Anti-Desmocollin 1 antibody [EPR8220] (ab150382) at 1/500000 dilution
Lane 1 : Empty vector transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : 293T cells transfected with myc-his tagged full length Desmocollin-1 expression vector, whole cell lysate
Lane 3 : 293T cells transfected with myc-his tagged full length Desmocollin-2 expression vector, whole cell lysate
Lane 4 : 293T cells transfected with myc-his tagged full length Desmocollin-3 expression vector, whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 7 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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![Anti-Desmocollin 1 antibody [EPR8220] (ab150382)](https://www.abcam.com/ps/products/150/ab150382/Images/ab150382-3-benefits-of-recombinant-antibodies.png)
Anti-Desmocollin 1 antibody [EPR8220] (ab150382)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab150382 has been referenced in 2 publications.
- Shuck SC et al. MLS128 antibody-induced suppression of colon cancer cell growth is mediated by a desmocollin and a 110 kDa glycoprotein. Biosci Trends 13:216-224 (2019). PubMed: 31168022
- Civciristov S et al. Ligand-dependent spatiotemporal signaling profiles of the µ-opioid receptor are controlled by distinct protein-interaction networks. J Biol Chem 294:16198-16213 (2019). PubMed: 31515267