Recombinant Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)

Thank you for contacting us. Although we do not have DSG 1 and 3 antibodies that have been tested for reactivity with horse or even in all the applications you anticipate using, we do have at least one DSG1 antibody that I think will be useful.

The catalogue number is ab124798:

Click here (or use the following: https://www.abcam.com/Desmoglein-1DSG1-antibody-EPR6766B-ab124798.html).

The immunogen sequence is 93% conserved in the horse sequence you sent (14 of 15 amino acids are identical), so it is likely that ab124798 will cross-react with the DSG1-like horse protein. The antibody has not been tested in IHC on frozen tissue sections, only paraffin-embedded tissue. It has not been tested by flow cytometry, but does work in immunocytochemistry, and is likely to work for flow cytometry as well.

For DSG3, the best candidate is ab14416, a mouse monoclonal raised against the extracellular portion of human DSG3. This region is 84% conserved in the horse sequence you sent. We do not know where the epitope that ab14416 recognizes falls within the extracellular region, so it is impossible to say if the epitope (generally consisting of a 6 - 8 amino acid sequence) is more or less than 84% identical to the homologous horse sequence. Again, the antibody has not been tested in all the applications you specified.

Click here (or use the following: https://www.abcam.com/Desmoglein-3PVA-antibody-5G11-ab14416.html).

I hope this helps. Please let me know if you have any questions.