Recombinant Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)
![Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)](https://www.abcam.com/ps/products/150/ab150372/Images/ab150372-447885-anti-desmoglein-2dsg2-antibody-epr6768-western-blot-hela-lysates.jpg "Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Desmoglein 2/DSG2 antibody [EPR6768]
See all Desmoglein 2/DSG2 primary antibodies -
Description
Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Monkey
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, HepG2, HeLa, Human fetal heart, mouse heart and rat heart lysates. IHC-P: Human thyroid gland papillary carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6768 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 122 kDa.
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| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Predicted molecular weight: 122 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
Tissue specificity
All of the tissues tested and carcinomas. -
Involvement in disease
Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Contains 4 cadherin domains. -
Domain
Calcium may be bound by the cadherin-like repeats. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
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Database links
- Entrez Gene: 1829 Human
- Entrez Gene: 13511 Mouse
- Entrez Gene: 307562 Rat
- Omim: 125671 Human
- SwissProt: Q14126 Human
- SwissProt: O55111 Mouse
- Unigene: 412597 Human
- Unigene: 345891 Mouse
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Alternative names
- ARVC 10 antibody
- ARVC10 antibody
- ARVD 10 antibody
see all
Images
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Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)All lanes : Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : DSG2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 90-160 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab150372 observed at 90-160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab150372 was shown to react with Desmoglein 2/DSG2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261826 (knockout cell lysate ab257158) was used. Wild-type HeLa and DSG2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab150372 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)](https://www.abcam.com/ps/products/150/ab150372/Images/ab150372-156952-anti-desmoglein-2-antibody-epr6768-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Desmoglein 2/DSG2 with ab150372 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)](https://www.abcam.com/ps/products/150/ab150372/Images/ab150372-414430-anti-desmoglein-2dsg2-antibody-epr6768-western-blot-hap1-scraped-control-hap1-scraped-hap1-dsg2-knockout-scraped-control-hap1-dsg2-knockout-scraped.jpg)
Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)All lanes : Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution
Lane 1 : Trypsinised HAP1 cell lysate
Lane 2 : Scraped HAP1 cell lysate
Lane 3 : Trypsinised DSG2 knockout HAP1 cell lysate
Lane 4 : Scraped DSG2 knockout HAP1 cell lysate
Lane 5 : Trypsinised A431 cell lysate
Lane 6 : Scraped A431 cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Lanes 1 - 6: Merged signal (red and green). Green - ab150372 observed at 95 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab150372 was shown to react with Desmoglein 2 in wild-type HAP1 cells in western blot with loss of signal observed in DSG2 knockout sample. Wild-type and DSG2 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab150372 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)](https://www.abcam.com/ps/products/150/ab150372/Images/ab150372-156953-anti-desmoglein-2-antibody-epr6768-western-blot.jpg)
Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)All lanes : Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart lysate
Lane 4 : mouse heart lysate
Lane 5 : rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 122 kDa -
![Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)](https://www.abcam.com/ps/products/150/ab150372/Images/ab150372-7-benefits-of-recombinant-antibodies.png)
Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab150372 has been referenced in 17 publications.
- Vallverdú-Prats M et al. Premature Termination Codon in 5' Region of Desmoplakin and Plakoglobin Genes May Escape Nonsense-Mediated Decay through the Reinitiation of Translation. Int J Mol Sci 23:N/A (2022). PubMed: 35054841
- Teng ACT et al. Tmem65 is critical for the structure and function of the intercalated discs in mouse hearts. Nat Commun 13:6166 (2022). PubMed: 36257954
- Yang L et al. Immune Profiling Reveals Molecular Classification and Characteristic in Urothelial Bladder Cancer. Front Cell Dev Biol 9:596484 (2021). PubMed: 33777927
- Qin S et al. DSG2 expression is correlated with poor prognosis and promotes early-stage cervical cancer. Cancer Cell Int 20:206 (2020). PubMed: 32514251
- Chen X et al. UPLC-Q-TOF/MS-Based Serum and Urine Metabonomics Study on the Ameliorative Effects of Palmatine on Helicobacter pylori-Induced Chronic Atrophic Gastritis. Front Pharmacol 11:586954 (2020). PubMed: 33041831