Recombinant Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)
![Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)](https://www.abcam.com/ps/products/109/ab109445/Images/ab109445-122306-anti-desmoplakin-antibody-epr43832-western-blot.jpg "Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4383(2)] to Desmoplakin
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Desmoplakin antibody [EPR4383(2)]
See all Desmoplakin primary antibodies -
Description
Rabbit monoclonal [EPR4383(2)] to Desmoplakin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, HACAT and BXPC-3 cell lysates. ICC/IF: A431 cells. Flow Cyt (intra): A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4383(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/250.
|
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| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 332 kDa.
|
| ICC/IF |
1/1000.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/250. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 332 kDa. |
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ICC/IF
1/1000. |
Target
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Relevance
Desmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. First, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Second, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. The desmosome intermediate filament complex is thought to impart tensile strength and resilience to the epithelium. Desmosomal proteins can be divided into 2 groups based on whether they fractionate with the urea-insoluble 'core' or the urea-soluble 'plaque' components. Desmoglein is, for example, a protein of the core. The main proteins of the plaque comprise the desmoplakins and plakoglobin. -
Cellular localization
Cell junction, desmosome. Cytoplasm, cytoskeleton. -
Database links
- Entrez Gene: 1832 Human
- Entrez Gene: 306871 Rat
- Omim: 125647 Human
- SwissProt: P15924 Human
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Alternative names
- 250/210 kDa paraneoplastic pemphigus antigen antibody
- Desmoplakin I antibody
- Desmoplakin II antibody
see all
Images
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Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)All lanes : Anti-Desmoplakin antibody [EPR4383(2)] (ab109445) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HACAT cell lysate
Lane 3 : BXPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 332 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)](https://www.abcam.com/ps/products/109/ab109445/Images/ab109445-261200-anti-desmoplakin-antibody-epr43832-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling Desmoplakin with ab109445 at 1/1000. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
![Flow Cytometry (Intracellular) - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)](https://www.abcam.com/ps/products/109/ab109445/Images/ab109445-287273-anti-desmoplakin-antibody-epr43832-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Desmoplakin (red) with ab109445 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)](https://www.abcam.com/ps/products/109/ab109445/Images/ab109445-5-benefits-of-recombinant-antibodies.png)
Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab109445 has been referenced in 9 publications.
- Yin L et al. KRT13 promotes stemness and drives metastasis in breast cancer through a plakoglobin/c-Myc signaling pathway. Breast Cancer Res 24:7 (2022). PubMed: 35078507
- Zhang C et al. Steroid receptor RNA activator inhibits the migration, invasion and stemness characteristics of renal cell carcinoma cells. Int J Mol Med 46:1765-1776 (2020). PubMed: 33000206
- Zhang R et al. Mechanical strain triggers differentiation of dental mesenchymal stem cells by activating osteogenesis-specific biomarkers expression. Am J Transl Res 11:233-244 (2019). PubMed: 30787982
- Ng R et al. Patient mutations linked to arrhythmogenic cardiomyopathy enhance calpain-mediated desmoplakin degradation. JCI Insight 5:N/A (2019). PubMed: 31194698
- Suga H et al. Keratinocyte Proline-Rich Protein Deficiency in Atopic Dermatitis Leads to Barrier Disruption. J Invest Dermatol 139:1867-1875.e7 (2019). PubMed: 30905808
- Ortega A et al. Intercalated disc in failing hearts from patients with dilated cardiomyopathy: Its role in the depressed left ventricular function. PLoS One 12:e0185062 (2017). PubMed: 28934278
- Hartlieb E et al. Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes. J Biol Chem 289:17043-53 (2014). WB . PubMed: 24782306
- Hartlieb E et al. Desmoglein 2 is less important than desmoglein 3 for keratinocyte cohesion. PLoS One 8:e53739 (2013). WB ; Human . PubMed: 23326495
- Tang H et al. CD133-positive cells might be responsible for efficient proliferation of human meningioma cells. Int J Mol Sci 13:6424-39 (2012). PubMed: 22754374