Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)

Submit a review Submit a question References (1)
Western blot - Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)
  • Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)
  • Flow Cytometry (Intracellular) - Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)
  • Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4383(2)] to Desmoplakin - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC PE Unconjugated

Overview

  • Product name

    Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free
    See all Desmoplakin primary antibodies

  • Description

    Rabbit monoclonal [EPR4383(2)] to Desmoplakin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247866 is the carrier-free version of ab109445.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 332 kDa.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 332 kDa.
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    Desmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. First, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Second, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. The desmosome intermediate filament complex is thought to impart tensile strength and resilience to the epithelium. Desmosomal proteins can be divided into 2 groups based on whether they fractionate with the urea-insoluble 'core' or the urea-soluble 'plaque' components. Desmoglein is, for example, a protein of the core. The main proteins of the plaque comprise the desmoplakins and plakoglobin.

  • Cellular localization

    Cell junction, desmosome. Cytoplasm, cytoskeleton.

  • Database links

    • Alternative names

      • 250/210 kDa paraneoplastic pemphigus antigen antibody
      • Desmoplakin I antibody
      • Desmoplakin II antibody
      • DP I antibody
      • DP II antibody
      • DPI antibody
      • DPII antibody
      • DSP antibody
      • KPPS2 antibody
      • PPKS2 antibody
      see all

    Images

    • Western blot - Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)
      Western blot - Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)
      All lanes : Anti-Desmoplakin antibody [EPR4383(2)] (ab109445) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HACAT cell lysate
      Lane 3 : BXPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 332 kDa



      This data was developed using ab109445, the same antibody clone in a different buffer formulation.

    • Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)
      Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)
      This data was developed using ab109445, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling Desmoplakin with ab109445 at 1/1000. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
      Control: PBS only.
      Nuclear counter stain: DAPI.
    • Flow Cytometry (Intracellular) - Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)
      Flow Cytometry (Intracellular) - Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)

      This data was developed using ab109445, the same antibody clone in a different buffer formulation. 

      Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Desmoplakin (red) with ab109445 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)
      Anti-Desmoplakin antibody [EPR4383(2)] - BSA and Azide free (ab247866)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab247866? Please let us know so that we can cite the reference in this datasheet.

    ab247866 has been referenced in 1 publication.

    • Wang X  et al. Paraneoplastic Pemphigus Autoantibodies Against C-terminus of Desmoplakin Induced Acantholysis In Vitro and In Vivo. Front Immunol 13:886226 (2022). PubMed: 35911677

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab247866.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com