Name: Anti-DGCR8 antibody [EPR18757]
Brand: RabMAb
SKU: ab191875
Rating: 5 (2 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-DGCR8 antibody [EPR18757] (ab191875)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18757] to DGCR8
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
Reacts with: Mouse, Rat, Human

APC Carrier Free

Product name

Anti-DGCR8 antibody [EPR18757]
See all DGCR8 primary antibodies
Description

Rabbit monoclonal [EPR18757] to DGCR8
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HEK-293, WEHI-3, Neuro-2a, PC-12 and NIH/3T3 whole cell lysates; Human fetal kidney lysate; Mouse brain, mouse testis and rat brain lysates. ICC/IF: HeLa and Jurkat cells. IP: HEK-293 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18757
Isotype

IgG
Research areas

Alternative Versions
- APC Anti-DGCR8 antibody [EPR18757] (ab221302)
- Anti-DGCR8 antibody [EPR18757] - BSA and Azide free (ab240325)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human DGCR8 protein (ab153373)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/1000.
WB	(2)	1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
ICC/IF		1/1000.
IP		1/60.

Notes
Flow Cyt (Intra) 1/1000.
WB 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
ICC/IF 1/1000.
IP 1/60.

Function

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.
Tissue specificity

Ubiquitously expressed.
Sequence similarities

Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 WW domain.
Domain

Both DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity.
Cellular localization

Nucleus. Nucleus > nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.
Target information above from: UniProt accession Q8WYQ5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 54487 Human
- Entrez Gene: 94223 Mouse
- Entrez Gene: 287954 Rat
- Omim: 609030 Human
- SwissProt: Q8WYQ5 Human
- SwissProt: Q9EQM6 Mouse
- Unigene: 643452 Human
- Unigene: 713579 Human
- Unigene: 447352 Mouse
- Unigene: 482156 Mouse
see all
Alternative names
- DGCRK6 antibody
- C22orf12 antibody
- D16H22S788E antibody
- D16Wis2 antibody
- DGCR 8 antibody
- Dgcr8 antibody
- DGCR8 microprocessor complex subunit antibody
- DGCR8_HUMAN antibody
- DGCRK 6 antibody
- DiGeorge syndrome critical region 8 antibody
- DiGeorge syndrome critical region gene 8 antibody
- Gy1 antibody
- Microprocessor complex subunit DGCR8 antibody
- pasha antibody
see all

Western blot - Anti-DGCR8 antibody [EPR18757] (ab191875)

All lanes : Anti-DGCR8 antibody [EPR18757] (ab191875) at 1/1000 dilution

Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : WEHI-3 (Mouse leukemia cell line) whole cell lysate
Lane 4 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
Lane 5 : Mouse testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-DGCR8 antibody [EPR18757] (ab191875)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling DGCR8 with purified ab191875 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor^® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-DGCR8 antibody [EPR18757] (ab191875)

Anti-DGCR8 antibody [EPR18757] (ab191875) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DGCR8 antibody [EPR18757] (ab191875)

All lanes : Anti-DGCR8 antibody [EPR18757] (ab191875) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DGCR8 antibody [EPR18757] (ab191875)

All lanes : Anti-DGCR8 antibody [EPR18757] (ab191875) at 1/1000 dilution

Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-DGCR8 antibody [EPR18757] (ab191875)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DGCR8 with ab191875 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab191875 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-DGCR8 antibody [EPR18757] (ab191875)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling DGCR8 with ab191875 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and weakly cytoplasmic staining on Jurkat cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab191875 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunoprecipitation - Anti-DGCR8 antibody [EPR18757] (ab191875)

DGCR8 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab191875 at 1/60 dilution.
Western blot was performed from the immunoprecipitate using ab191875 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 whole cell lysate 10ug (Input).
Lane 2: ab191875 IP in HEK-293 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191875 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Anti-DGCR8 antibody [EPR18757] (ab191875)

Flow cytometry protocols
Immunoprecipitation protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab191875? Please let us know so that we can cite the reference in this datasheet.

ab191875 has been referenced in 25 publications.

Zhang S et al. SPI1-induced downregulation of FTO promotes GBM progression by regulating pri-miR-10a processing in an m6A-dependent manner. Mol Ther Nucleic Acids 27:699-717 (2022). PubMed: 35317283
Reé D et al. Partial Disturbance of Microprocessor Function in Human Stem Cells Carrying a Heterozygous Mutation in the DGCR8 Gene. Genes (Basel) 13:N/A (2022). PubMed: 36360162
Hang Q et al. Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance. Nat Commun 12:4033 (2021). PubMed: 34188037
Song H et al. SPINT1-AS1 Drives Cervical Cancer Progression via Repressing miR-214 Biogenesis. Front Cell Dev Biol 9:691140 (2021). PubMed: 34350182
Gao J et al. Merkel cell polyomavirus T-antigens regulate DICER1 mRNA stability and translation through HSC70. iScience 24:103264 (2021). PubMed: 34761184

View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review

1-2 of 2 Abreviews

Application

Western blot

Sample

Zebrafish Tissue lysate - whole (ovarian follicles)

Gel Running Conditions

Reduced Denaturing (15%)

Loading amount

20 µg

Treatment

ZnCl2, ZnSO4, TPEN, Testosterone, Testosterone+TPEN for 12h

Specification

ovarian follicles

Blocking step

BSA+Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 31 2019

Application

Western blot

Sample

Human Cell lysate - nuclear (A549 cells)

Gel Running Conditions

Reduced Denaturing (8%)

Loading amount

35 µg

Specification

A549 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Wei-Ting Lu

Verified customer

Submitted May 09 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (25)

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Western blot abreview for Anti-DGCR8 antibody [EPR18757]

Western blot abreview for Anti-DGCR8 antibody [EPR18757]