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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Dicer antibody [EPR24104-105] (ab259327)

Submit a review Submit a question References (2)
Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)
  • Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)
  • Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)
  • Anti-Dicer antibody [EPR24104-105] (ab259327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24104-105] to Dicer
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Dicer antibody [EPR24104-105]
    See all Dicer primary antibodies

  • Description

    Rabbit monoclonal [EPR24104-105] to Dicer

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt (Intra),ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW264.7, 2.4G2, HeLa, 293T and NIH/3T3, PC-12 lysates; WT HAP1. IHC-P: Human testis, Mouse cerebrum and testis, Rat testis tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab259327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 218 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Predicted molecular weight: 218 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

Target

  • Function

    Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.

  • Involvement in disease

    Pleuropulmonary blastoma
    Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
    Rhabdomyosarcoma, embryonal, 2
    DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).

  • Sequence similarities

    Belongs to the helicase family. Dicer subfamily.
    Contains 1 Dicer dsRNA-binding fold domain.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PAZ domain.
    Contains 2 RNase III domains.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DCR antibody
    • DCR1 antibody
    • Dicer 1 ribonuclease III antibody
    • Dicer 1 ribonuclease type III antibody
    • Dicer antibody
    • DICER_HUMAN antibody
    • DICER1 antibody
    • Double-strand-specific ribonuclease antibody
    • Endoribonuclease Dicer antibody
    • Helicase MOI antibody
    • Helicase with RNase motif antibody
    • HERNA antibody
    • KIAA0928 antibody
    • MNG1 antibody
    • RMSE2 antibody
    see all

Images

  • Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)
    Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)
    All lanes : Anti-Dicer antibody [EPR24104-105] (ab259327) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate
    Lane 2 : Dicer knockout HAP1 whole cell lysate
    Lane 3 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 4 : 2.4G2 (rat B cell lymphoma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

    Predicted band size: 218 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab259327 observed at 250 kDa. Red - Loading Control ab7291 observed at 50 kDa.

    ab259327 was shown to specifically react with Dicer in wild-type HAP1 cells. Loss of signal was observed when Dicer knockout samples were used. Wild-type and Dicer knockout samples were subjected to SDS-PAGE. ab259327 and anti-alpha Tubulin - Loading Control ( ab7291) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)
    Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)
    All lanes : Anti-Dicer antibody [EPR24104-105] (ab259327) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : 2.4G2 (rat B cell lymphoma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 218 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: THP-1 (PMID:20584909).

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

     

     Exposure time: 48 seconds

     

  • Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)
    Western blot - Anti-Dicer antibody [EPR24104-105] (ab259327)
    All lanes : Anti-Dicer antibody [EPR24104-105] (ab259327) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 218 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

    Exposure time: Lane 1, 3, 4: 3 minutes

    Lane 2: 26 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID:17573847). The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID:32737764). The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis. The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [EPR24104-105] (ab259327)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling Dicer with ab259327 at 1/100 dilution (5.05 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis. The section was incubated with ab259327 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Anti-Dicer antibody [EPR24104-105] (ab259327)
    Anti-Dicer antibody [EPR24104-105] (ab259327)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab259327? Please let us know so that we can cite the reference in this datasheet.

ab259327 has been referenced in 2 publications.

  • Antoniali G  et al. APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b. Cell Mol Life Sci 79:446 (2022). PubMed: 35876890
  • Wang J  et al. MiR-186 Suppressed Growth, Migration, and Invasion of Lung Adenocarcinoma Cells via Targeting Dicer1. J Oncol 2021:6217469 (2021). PubMed: 34804161

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