Recombinant Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
![Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)](https://www.abcam.com/ps/products/124/ab124933/Images/ab124933-253051-anti-dishevelled-2-phospho-s143-antibody-epncir145-western-blot.jpg "Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143)
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145]
See all Dishevelled 2 primary antibodies -
Description
Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab173138) -
Positive control
- WB: F9 and HEK293 cell lysates.
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR145 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).Can be blocked with Dishevelled 2 peptide (ab173138).
For unpurified use at 1/1000 - 1/10000. |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).Can be blocked with Dishevelled 2 peptide (ab173138). For unpurified use at 1/1000 - 1/10000. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. -
Sequence similarities
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
Domain
The DIX domain mediates homooligomerization. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta. - Information by UniProt
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Database links
- Entrez Gene: 1856 Human
- Entrez Gene: 13543 Mouse
- Omim: 602151 Human
- SwissProt: O14641 Human
- SwissProt: Q60838 Mouse
- Unigene: 118640 Human
- Unigene: 5114 Mouse
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Alternative names
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
see all
Images
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Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (purified)
Lane 1 : Untreated F9 cell lysate
Lane 2 : Alkaline phosphatase treated F9 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)](https://www.abcam.com/ps/products/124/ab124933/Images/ab124933-253050-anti-dishevelled-2-phospho-s143-antibody-epncir145-western-blot.jpg)
Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/5000 dilution (purified)
Lane 1 : Untreated HEK293 cell lysate
Lane 2 : Alkaline phosphatase treated HEK293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)](https://www.abcam.com/ps/products/124/ab124933/Images/ab124933-138963-anti-dishevelled-2-phospho-s143-antibody-epncir145-western-blot.jpg)
Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (unpurified)
Lane 1 : F9 cell lysate untreated
Lane 2 : F9 cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
![Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)](https://www.abcam.com/ps/products/124/ab124933/Images/ab124933-4-benefits-of-recombinant-antibodies.png)
Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab124933 has been referenced in 10 publications.
- Mun H et al. The Autophagy Regulator p62 Controls PTEN-Dependent Ciliogenesis. Front Cell Dev Biol 8:465 (2020). PubMed: 32587859
- Peng Y & Wang HH Cir-ITCH inhibits gastric cancer migration, invasion and proliferation by regulating the Wnt/ß-catenin pathway. Sci Rep 10:17443 (2020). PubMed: 33060778
- Endo M et al. E2F1-Ror2 signaling mediates coordinated transcriptional regulation to promote G1/S phase transition in bFGF-stimulated NIH/3T3 fibroblasts. FASEB J 34:3413-3428 (2020). PubMed: 31922321
- Mei J et al. Nerve modulation therapy in gouty arthritis: targeting increased sFRP2 expression in dorsal root ganglion regulates macrophage polarization and alleviates endothelial damage. Theranostics 9:3707-3722 (2019). PubMed: 31281508
- Luo Y et al. Atypical function of a centrosomal module in WNT signalling drives contextual cancer cell motility. Nat Commun 10:2356 (2019). PubMed: 31142743
- Jin C et al. The CK2 inhibitor CX4945 reverses cisplatin resistance in the A549/DDP human lung adenocarcinoma cell line. Oncol Lett 18:3845-3856 (2019). PubMed: 31579410
- Xie J et al. PP5 (PPP5C) is a phosphatase of Dvl2. Sci Rep 8:2715 (2018). PubMed: 29426949
- Song Z et al. PTEN silencing enhances neuronal proliferation and differentiation by activating PI3K/Akt/GSK3ß pathway in vitro. Exp Cell Res 363:179-187 (2018). PubMed: 29305963
- Moreno-Fortuny A et al. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open 6:1592-1601 (2017). PubMed: 28923978
- Shnitsar I et al. PTEN regulates cilia through Dishevelled. Nat Commun 6:8388 (2015). WB . PubMed: 26399523