Recombinant Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941)
![Western blot - Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941)](https://www.abcam.com/ps/products/124/ab124941/Images/ab124941-138982-anti-dishevelled-2-phospho-t224-antibody-epncir146-western-blot.jpg "Western blot - Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR146] to Dishevelled 2 (phospho T224)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146]
See all Dishevelled 2 primary antibodies -
Description
Rabbit monoclonal [EPNCIR146] to Dishevelled 2 (phospho T224) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cell lysate treated with Calyculin A
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR146 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. -
Sequence similarities
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
Domain
The DIX domain mediates homooligomerization. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta. - Information by UniProt
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Database links
- Entrez Gene: 1856 Human
- Entrez Gene: 13543 Mouse
- Entrez Gene: 303251 Rat
- Omim: 602151 Human
- SwissProt: O14641 Human
- SwissProt: Q60838 Mouse
- Unigene: 118640 Human
- Unigene: 5114 Mouse
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Alternative names
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
see all
Images
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Western blot - Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941)All lanes : Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941) at 1/1000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
![Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941)](https://www.abcam.com/ps/products/124/ab124941/Images/ab124941-2-benefits-of-recombinant-antibodies.png)
Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941)
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab124941 has been referenced in 4 publications.
- Esaki N et al. The Daple-CK1e complex regulates Dvl2 phosphorylation and canonical Wnt signaling. Biochem Biophys Res Commun 532:406-413 (2020). PubMed: 32888647
- Endo M et al. E2F1-Ror2 signaling mediates coordinated transcriptional regulation to promote G1/S phase transition in bFGF-stimulated NIH/3T3 fibroblasts. FASEB J 34:3413-3428 (2020). PubMed: 31922321
- Jin C et al. The CK2 inhibitor CX4945 reverses cisplatin resistance in the A549/DDP human lung adenocarcinoma cell line. Oncol Lett 18:3845-3856 (2019). PubMed: 31579410
- Eubelen M et al. A molecular mechanism for Wnt ligand-specific signaling. Science 361:N/A (2018). PubMed: 30026314