Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)
Key features and details
- Rabbit monoclonal [EP1991Y] to Dishevelled 3
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Rat, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-Dishevelled 3 antibody [EP1991Y]
See all Dishevelled 3 primary antibodies -
Description
Rabbit monoclonal [EP1991Y] to Dishevelled 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562 cell lysate IHC-P: Human brain tissue ICC/IF: PC12 cells Flow Cyt: HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1991Y -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 78 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 5 µg/ml.
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|
Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 78 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
May play a role in the signal transduction pathway mediated by multiple Wnt genes. -
Sequence similarities
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsUbiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1857 Human
- Entrez Gene: 13544 Mouse
- Entrez Gene: 303811 Rat
- Omim: 601368 Human
- SwissProt: Q92997 Human
- SwissProt: Q61062 Mouse
- Unigene: 388116 Human
- Unigene: 247259 Mouse
see all -
Alternative names
- Dishevelled 3 (homologous to Drosophila dsh) antibody
- Dishevelled 3 antibody
- Dishevelled 3, dsh homolog (Drosophila) antibody
see all
Images
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Anti-Dishevelled 3 antibody [EP1991Y] (ab76081) at 1/1000 dilution + K562 cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labelled IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
Immunohistochemical staining of paraffin-embedded human brain tissue, using ab76081, at 1/100-1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF image of ab76081 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76081, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing HeLa cells stained with ab76081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76081, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab76081 has been referenced in 8 publications.
- Asif M et al. De novo variants of CSNK2B cause a new intellectual disability-craniodigital syndrome by disrupting the canonical Wnt signaling pathway. HGG Adv 3:100111 (2022). PubMed: 35571680
- Pruller J et al. DVL1 and DVL3 require nuclear localisation to regulate proliferation in human myoblasts. Sci Rep 12:8388 (2022). PubMed: 35589804
- Zeng X et al. Secreted Frizzled Related Protein 2 Modulates Epithelial-Mesenchymal Transition and Stemness via Wnt/ß-Catenin Signaling in Choriocarcinoma. Cell Physiol Biochem 50:1815-1831 (2018). PubMed: 30396168
- Hua Y et al. Oligomerization of Frizzled and LRP5/6 protein initiates intracellular signaling for the canonical WNT/ß-catenin pathway. J Biol Chem 293:19710-19724 (2018). PubMed: 30361437
- Wu Y et al. Low expression of secreted frizzled-related protein 2 and nuclear accumulation of ß-catenin in aggressive nonfunctioning pituitary adenoma. Oncol Lett 12:199-206 (2016). WB ; Human . PubMed: 27347125
- He H et al. miR-204-5p promotes the adipogenic differentiation of human adipose-derived mesenchymal stem cells by modulating DVL3 expression and suppressing Wnt/ß-catenin signaling. Int J Mol Med 35:1587-95 (2015). WB . PubMed: 25847080
- Lin X et al. Differential Expression of Wnt Pathway Genes in Sporadic Hepatocellular Carcinomas Infected With Hepatitis B Virus Identified With OligoGE Arrays. Hepat Mon 13:e6192 (2013). PubMed: 23483081
- Burcklé C et al. Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros. Hum Mol Genet 20:2611-27 (2011). IHC (PFA fixed) . PubMed: 21498478