Anti-DLST antibody [9F4BD5] (ab110306)
![Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)](https://www.abcam.com/ps/products/110/ab110306/Images/ab110306-123574-anti-dlst-antibody-9f4bd5-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)")
Key features and details
- Mouse monoclonal [9F4BD5] to DLST
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-DLST antibody [9F4BD5]
See all DLST primary antibodies -
Description
Mouse monoclonal [9F4BD5] to DLST -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Human HDFn and HL-60 cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The purity of ab110306 is near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
9F4BD5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| Flow Cyt | (1) |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). -
Pathway
Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6. -
Sequence similarities
Belongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 1743 Human
- Omim: 126063 Human
- SwissProt: P36957 Human
- Unigene: 525459 Human
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Alternative names
- 2-oxoglutarate dehydrogenase complex component E2 antibody
- Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
- Dihydrolipoamide S succinyltransferase antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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![Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)](https://www.abcam.com/ps/products/110/ab110306/Images/ab110306-123573-anti-dlst-antibody-9f4bd5-flow-cytometry.jpg)
Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)HL-60 cells were stained with 1 µg/mL ab110306 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry. -
![Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)](https://www.abcam.com/ps/products/110/ab110306/Images/ab110306-123572-anti-dlst-antibody-9f4bd5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreviewab110306 staining DLST in Human monocyte-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% Goat serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 12 hours at 4°C. A Cy2®-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody. -
![Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)](https://www.abcam.com/ps/products/110/ab110306/Images/ab110306-123571-anti-dlst-antibody-9f4bd5-flow-cytometry.jpg)
Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreviewab110306 staining DLST in Human monocyte-derived macrophages by Flow Cytometry. Cells were prepared by scraoing in PBS and fixation by paraformaldehyde. The sample was incubated with the primary antibody (1/1000 in PBS + 1% BSA) for 60 minutes at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/100) was used as the secondary antibody.
Gating Strategy: Dead cells excluded.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab110306 has been referenced in 2 publications.
- Leandro J et al. DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo. Hum Mol Genet 29:1168-1179 (2020). PubMed: 32160276
- Malty RH et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). PubMed: 29128334