Anti-DNA polymerase alpha/POLA antibody (ab31777)

Thank you for your patience.
For ab57296, the sequence homology of the immunogen with mouse is 83% - which is good.
The lab let me know that for ab65009, the amino acid range of the immunogen is within residues 100-300 of human DNA Polymerase alpha with UniProt accession number P09884. The alignment of the immunogen sequence with the mouse protein (P33609) gives only a 61.5% sequence identity.
We usually say that a homology of 85% or higher is best for cross-reactivity. Although the immunogen region is 50 residues, the actual epitope of the antibody binding site is only 5-10 residues. This could mean the actual homology for the epitope could be either better than 61.5% or could also be worse. This is very difficult to predict and would need to be tried out.
Since none of our Anti-DNA polymerase alpha antibody has been tested in mouse, you would be eligible for our testing discount program.
For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the to-be-tested product at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount in the amount of the price you paid for the to-be-tested product. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.
Please let me know if you would like to try any of the antibody and if I should send you a discount code.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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