Recombinant Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)
![Flow Cytometry (Intracellular) - Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)](https://www.abcam.com/ps/products/157/ab157216/Images/ab157216-164418-anti-dnaja2-antibody-epr11302b-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11302(B)] to DNAJA2
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DNAJA2 antibody [EPR11302(B)]
See all DNAJA2 primary antibodies -
Description
Rabbit monoclonal [EPR11302(B)] to DNAJA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, MCF7, Jurkat and K562 cell lysates. Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900). K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11302(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50000 - 1/200000. Predicted molecular weight: 46 kDa.
|
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/50000 - 1/200000. Predicted molecular weight: 46 kDa. |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Co-chaperone of Hsc70. -
Sequence similarities
Contains 1 CR-type zinc finger.
Contains 1 J domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10294 Human
- Entrez Gene: 56445 Mouse
- Entrez Gene: 84026 Rat
- Omim: 611322 Human
- SwissProt: O60884 Human
- SwissProt: Q9QYJ0 Mouse
- SwissProt: O35824 Rat
- Unigene: 368078 Human
see all -
Alternative names
- Cell cycle progression 3 protein antibody
- Cell cycle progression restoration gene 3 protein antibody
- CPR 3 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)
Intracellular flow cytometric analysis of permeabilized K562 cells labeling DNAJA2 with either ab157216 at 1/10 dilution (red) or a Rabbit IgG negative control (green).
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![Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)](https://www.abcam.com/ps/products/157/ab157216/Images/ab157216-164417-anti-dnaja2-antibody-epr11302b-western-blot.jpg)
Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)All lanes : Anti-DNAJA2 antibody [EPR11302(B)] (ab157216) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa -
![Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)](https://www.abcam.com/ps/products/157/ab157216/Images/ab157216-164416-anti-dnaja2-antibody-epr11302b-western-blot.jpg)
Western blot - Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)Anti-DNAJA2 antibody [EPR11302(B)] (ab157216) at 1/50000 dilution + Immunoprecipitation pellet from HepG2 cell lysate at 10 µg
Predicted band size: 46 kDa -
![Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)](https://www.abcam.com/ps/products/157/ab157216/Images/ab157216-4-benefits-of-recombinant-antibodies.png)
Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab157216 has been referenced in 1 publication.
- Nillegoda NB et al. Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes. Elife 6:N/A (2017). PubMed: 28504929