Anti-Dnmt1 antibody [60B1220.1] (ab13537)
![Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)](https://www.abcam.com/ps/products/13/ab13537/Images/ab13537-397614-AntiDnmt1-antibody-60B12201-western-blot-H1299-human.jpg "Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)")
Key features and details
- Mouse monoclonal [60B1220.1] to Dnmt1
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Dnmt1 antibody [60B1220.1]
See all Dnmt1 primary antibodies -
Description
Mouse monoclonal [60B1220.1] to Dnmt1 -
Host species
Mouse -
Specificity
This antibody detects a ~180 kDa protein, corresponding to the apparent molecular mass of Dnmt1 on SDS-PAGE immunoblots in samples of human and mouse origin. Immunogen itself has been shown to be toxic. -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide:
EKDDREDKENAFKR
, corresponding to amino acids 637-650 of Human Dnmt1 -
Positive control
- WB: H1299 cell lysate. IHC-Fr: Human colon carcinoma tissue. Mouse medullar kidney tissue. Flow Cyt: HeLa cells.
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General notes
For maximum product recovery, centrifuge the product vial before removing cap. Shipped at on gel packs.
DNMT1 high levels are toxic, as a result it may be tough to find sometimes. Signal amplification might be needed.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody is affinity purified. -
Clonality
Monoclonal -
Clone number
60B1220.1 -
Isotype
IgG1 -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| WB | (12) |
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa).
Block in 1.5% BSA in 1x TBST. Other Band(s): Lower bands are DNMT1 degradation. |
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Block in 1.5% BSA in 1x TBST. Other Band(s): Lower bands are DNMT1 degradation. |
Target
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Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. -
Tissue specificity
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger. -
Domain
The N-terminal part is required for homodimerization and acts as a regulatory domain. -
Post-translational
modificationsSumoylated; sumoylation increases activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281119 Cow
- Entrez Gene: 1786 Human
- Entrez Gene: 13433 Mouse
- Omim: 126375 Human
- SwissProt: Q24K09 Cow
- SwissProt: P26358 Human
- SwissProt: P13864 Mouse
- Unigene: 202672 Human
see all -
Alternative names
- ADCADN antibody
- AIM antibody
- CXXC finger protein 9 antibody
see all
Images
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Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)Anti-Dnmt1 antibody [60B1220.1] (ab13537) at 1/1000 dilution + H1299 (Human non-small cell lung carcinoma cell line) lysate
Predicted band size: 180 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)](https://www.abcam.com/ps/products/13/ab13537/Images/ab13537-20840-anti-dnmt1-antibody-60b12201-chip-grade-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)Immunohistochemistry analysis of formalin-fixed human colon carcinoma tissue labelling Dnmt1 with ab13537 at 1/10000 dilution (12 hours at 4°C), followed by biotin Goat Anti-Mouse antibody at 1/2000 for 1 hour at RT. Counterstain: Hematoxylin (purple/blue) nuclear stain at 200µl for 2 mins at RT.
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![Flow Cytometry - Anti-Dnmt1 antibody [60B1220.1] (ab13537)](https://www.abcam.com/ps/products/13/ab13537/Images/ab13537-20839-anti-dnmt1-antibody-60b12201-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-Dnmt1 antibody [60B1220.1] (ab13537)Overlay histogram showing HeLa cells stained with ab13537 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13537, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)](https://www.abcam.com/ps/products/13/ab13537/Images/ab13537-308658-anti-dnmt1-antibody-60b12201-chip-grade-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] (ab13537)Immunohistochemistry analysis of mouse medullar kidney tissue labelling Dnmt1 with ab13537 at 1/10000 dilution, followed by biotin Goat Anti-Mouse antibody (brown). Counterstain: Hematoxylin (purple/blue) nuclear stain.
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![Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)](https://www.abcam.com/ps/products/13/ab13537/Images/ab13537-20837-anti-dnmt1-antibody-60b12201-chip-grade-western-blot.jpg)
Western blot - Anti-Dnmt1 antibody [60B1220.1] (ab13537)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Dnmt1 antibody [60B1220.1] (ab13537) at 1 µg/ml
Lane 1 : HCT116 whole cell lysate
Lane 2 : HCT116 DNMT1 KO whole cell lysate
Lane 3 : HCT116 DNMT3b KO whole cell lysate
Secondary
All lanes : HRP conjugated Sheep anti-mouse IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Exposure time: 1 minute
Datasheets and documents
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SDS download
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Datasheet download
References (175)
ab13537 has been referenced in 175 publications.
- Wang Y et al. Epigenetic regulation of intestinal peptide transporter PEPT1 as a potential strategy for colorectal cancer sensitization. Cell Death Dis 12:532 (2021). WB ; Human . PubMed: 34031358
- González-Rodríguez P et al. The DNA methyltransferase DNMT3A contributes to autophagy long-term memory. Autophagy 17:1259-1277 (2021). PubMed: 32876528
- Seo SI et al. HOTAIR Induces Methylation of PCDH10, a Tumor Suppressor Gene, by Regulating DNMT1 and Sponging with miR-148b in Gastric Adenocarcinoma. Yonsei Med J 62:118-128 (2021). PubMed: 33527791
- Ma T et al. HOXA10 promotion of HDAC1 underpins the development of lung adenocarcinoma through the DNMT1-KLF4 axis. J Exp Clin Cancer Res 40:71 (2021). PubMed: 33596966
- Shao G et al. Methylation-dependent MCM6 repression induced by LINC00472 inhibits triple-negative breast cancer metastasis by disturbing the MEK/ERK signaling pathway. Aging (Albany NY) 13:4962-4975 (2021). PubMed: 33668040