Recombinant Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (ab313857)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL1478] to Dopamine D2 Receptor - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free
See all Dopamine D2 Receptor primary antibodies -
Description
Rabbit monoclonal [HL1478] to Dopamine D2 Receptor - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Dopamine D2 Receptor (N terminal).
Database link: P14416 -
Positive control
- WB: Mouse brain tissue extract. Unboiled SH-SY5Y whole cell extract and membrane extract. ICC/IF: DIV9 rat E18 primary cortical neurons. SH-SY5Y cells. IHC-P: Rat brain tissue.
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General notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL1478 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab313857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 50.619 kDa.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 50.619 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. -
Involvement in disease
Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1813 Human
- Entrez Gene: 13489 Mouse
- Entrez Gene: 24318 Rat
- Omim: 126450 Human
- SwissProt: P14416 Human
- SwissProt: P61168 Mouse
- SwissProt: P61169 Rat
- Unigene: 73893 Human
see all -
Alternative names
- D(2) dopamine receptor antibody
- D2 dopamine receptor antibody
- D2DR antibody
see all
Images
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All lanes : Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (ab313857) at 1/1000 dilution
Lane 1 : Unboiled SH-SY5Y SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell extract (WCE)
Lane 2 : Unboiled SH-SY5Y membrane extract (ME)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50.619 kDa -
All lanes : Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (ab313857) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Mouse spleen tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50.619 kDa -
Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells fixed in ice-cold MeOH for 5 minutes labeling Dopamine Receptor D2 with ab313857 at 1/1000 dilution (green). Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.
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Immunofluorescent analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling Dopamine Receptor D2 with ab313857 at 1/250 dilution (green). Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1/500 dilution. Blue: Fluoroshield with DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling Dopamine Receptor D2 with ab313857 at 1/100 dilution. Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab313857 has not yet been referenced specifically in any publications.