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Recombinant

Recombinant Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (ab313857)

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Western blot - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
  • Western blot - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL1478] to Dopamine D2 Receptor - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free
    See all Dopamine D2 Receptor primary antibodies

  • Description

    Rabbit monoclonal [HL1478] to Dopamine D2 Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Human Dopamine D2 Receptor (N terminal).
    Database link: P14416

  • Positive control

    • WB: Mouse brain tissue extract. Unboiled SH-SY5Y whole cell extract and membrane extract. ICC/IF: DIV9 rat E18 primary cortical neurons. SH-SY5Y cells. IHC-P: Rat brain tissue.

  • General notes

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab313857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50.619 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50.619 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

  • Involvement in disease

    Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

Images

  • Western blot - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
    Western blot - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
    All lanes : Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (ab313857) at 1/1000 dilution

    Lane 1 : Unboiled SH-SY5Y SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell extract (WCE)
    Lane 2 : Unboiled SH-SY5Y membrane extract (ME)

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 50.619 kDa

  • Western blot - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
    Western blot - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
    All lanes : Anti-Dopamine D2 Receptor antibody [HL1478] - BSA and Azide free (ab313857) at 1/1000 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Mouse spleen tissue extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 50.619 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
    Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)

    Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells fixed in ice-cold MeOH for 5 minutes labeling Dopamine Receptor D2 with ab313857 at 1/1000 dilution (green). Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.

  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
    Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)

    Immunofluorescent analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling Dopamine Receptor D2 with ab313857 at 1/250 dilution (green). Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1/500 dilution. Blue: Fluoroshield with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine D2 Receptor antibody [HL1478] (ab313857)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling Dopamine Receptor D2 with ab313857 at 1/100 dilution. Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab313857? Please let us know so that we can cite the reference in this datasheet.

ab313857 has not yet been referenced specifically in any publications.

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