Anti-Dopamine Receptor D1 antibody (ab40653)
Key features and details
- Rabbit polyclonal to Dopamine Receptor D1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Dopamine Receptor D1 antibody
See all Dopamine Receptor D1 primary antibodies -
Description
Rabbit polyclonal to Dopamine Receptor D1 -
Host species
Rabbit -
Specificity
This antibody shows reactivity with: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0%; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; and D5 Dopamine Receptor 0%. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Pig, Macaque monkey, Rhesus monkey
-
Immunogen
Synthetic peptide corresponding to Human Dopamine Receptor D1 aa 1-100 (Cysteine residue).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Lyophilized:Reconstitute with 0.1ml of PBS which contains 10 mg/ml BSA. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| WB |
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
|
|
| IHC-P |
1/500.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
|
IHC-P
1/500. |
Target
-
Function
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
Tissue specificity
Detected in caudate, nucleus accumbens and in the olfactory tubercle. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. - Information by UniProt
-
Database links
- Entrez Gene: 281125 Cow
- Entrez Gene: 1812 Human
- Entrez Gene: 13488 Mouse
- Entrez Gene: 24316 Rat
- Omim: 126449 Human
- SwissProt: Q95136 Cow
- SwissProt: P21728 Human
- SwissProt: Q61616 Mouse
see all -
Alternative names
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody (ab40653)ab40653 (1:500) staining Dopamine Receptor D1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic and membrane regions in the fibrous glial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Western blot - Anti-Dopamine Receptor D1 antibody (ab40653)All lanes : Anti-Dopamine Receptor D1 antibody (ab40653) at 1/1000 dilution
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 42 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
Protocols
Datasheets and documents
-
Datasheet download
References (15)
ab40653 has been referenced in 15 publications.
- Gao S et al. Striatal D1 Dopamine Neuronal Population Dynamics in a Rat Model of Levodopa-Induced Dyskinesia. Front Aging Neurosci 14:783893 (2022). PubMed: 35185524
- Agustí A et al. Bacteroides uniformis CECT 7771 Modulates the Brain Reward Response to Reduce Binge Eating and Anxiety-Like Behavior in Rat. Mol Neurobiol 58:4959-4979 (2021). PubMed: 34228269
- Felippe RM et al. Experimental Social Stress: Dopaminergic Receptors, Oxidative Stress, and c-Fos Protein Are Involved in Highly Aggressive Behavior. Front Cell Neurosci 15:696834 (2021). PubMed: 34489642
- Eom TY et al. Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun 11:912 (2020). PubMed: 32060266
- Williams MT et al. Whole brain proton irradiation in adult Sprague Dawley rats produces dose dependent and non-dependent cognitive, behavioral, and dopaminergic effects. Sci Rep 10:21584 (2020). PubMed: 33299021
