Recombinant Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-253826-anti-dopamine-receptor-d1-antibody-ep1560y-western-blot.jpg "Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1
- Suitable for: WB
- Reacts with: Mouse, Rat, Guinea pig, Human
Related conjugates and formulations
Overview
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Product name
Anti-Dopamine Receptor D1 antibody [EP1560Y]
See all Dopamine Receptor D1 primary antibodies -
Description
Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Guinea pig, Human -
Immunogen
Synthetic peptide within Human Dopamine Receptor D1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P21728 -
Positive control
- WB: SH-SY5Y cell lysate, human fetal kidney, guinea pig brain, mouse and rat brain and kidney tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1560Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Mouse kidney tissue lysate - total protein (0 days) (ab7261)
- Mouse kidney tissue lysate (14 days) (ab7262)
- Mouse kidney tissue lysate (7 days) (ab7263)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa. |
Application notes
Is unsuitable for Flow Cyt or ICC/IF.
Target
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Function
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
Tissue specificity
Detected in caudate, nucleus accumbens and in the olfactory tubercle. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. - Information by UniProt
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Database links
- Entrez Gene: 1812 Human
- Entrez Gene: 13488 Mouse
- Entrez Gene: 24316 Rat
- Omim: 126449 Human
- SwissProt: P21728 Human
- SwissProt: Q61616 Mouse
- SwissProt: P18901 Rat
- Unigene: 2624 Human
see all -
Alternative names
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
Images
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Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)All lanes : Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : Guinea pig brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 (ab81296) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-253825-anti-dopamine-receptor-d1-antibody-ep1560y-western-blot.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/5000 dilution (purified) + SH-SY5Y whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-253824-anti-dopamine-receptor-d1-antibody-ep1560y-western-blot.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-96501-anti-dopamine-receptor-d1-antibody-ep1560y-western-blot.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-6-benefits-of-recombinant-antibodies.png)
Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab81296 has been referenced in 16 publications.
- Wu M et al. Electroacupuncture Alleviates Anxiety-Like Behaviors Induced by Chronic Neuropathic Pain via Regulating Different Dopamine Receptors of the Basolateral Amygdala. Mol Neurobiol 59:5299-5311 (2022). PubMed: 35696012
- Qian X et al. Dopamine Pathway Mediated by DRD5 Facilitates Tumor Growth via Enhancing Warburg Effect in Esophageal Cancer. Front Oncol 11:655861 (2021). PubMed: 33898321
- Tavares G et al. Dopamine D2 receptor agonist, bromocriptine, remodels adipose tissue dopaminergic signalling and upregulates catabolic pathways, improving metabolic profile in type 2 diabetes. Mol Metab N/A:101241 (2021). PubMed: 33933677
- Liu G et al. Inactivation of Cys674 in SERCA2 increases BP by inducing endoplasmic reticulum stress and soluble epoxide hydrolase. Br J Pharmacol 177:1793-1805 (2020). PubMed: 31758704
- Lin CC et al. Early life social experience affects adulthood fear extinction deficit and associated dopamine profile abnormalities in a rat model of PTSD. Prog Neuropsychopharmacol Biol Psychiatry 101:109914 (2020). PubMed: 32165120