Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021)
![Western blot - Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021)](https://www.abcam.com/ps/products/78/ab78021/Images/ab78021-93805-anti-dopamine-receptor-d1-antibody-sg2-d1a-western-blot.jpg "Western blot - Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021)")
Key features and details
- Mouse monoclonal [SG2-D1a] to Dopamine Receptor D1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2b
Overview
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Product name
Anti-Dopamine Receptor D1 antibody [SG2-D1a]
See all Dopamine Receptor D1 primary antibodies -
Description
Mouse monoclonal [SG2-D1a] to Dopamine Receptor D1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Sheep
Does not react with:
Rabbit, Cow, Dog, Human, Pig -
Immunogen
Recombinant fragment corresponding to Rat Dopamine Receptor D1 (C terminal).
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Positive control
- Mouse brain lysate; rat caudate tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SG2-D1a -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/250 - 1/500. Predicted molecular weight: 49 kDa.
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| Notes |
|---|
|
WB
1/250 - 1/500. Predicted molecular weight: 49 kDa. |
Target
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Function
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
Tissue specificity
Detected in caudate, nucleus accumbens and in the olfactory tubercle. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. - Information by UniProt
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Database links
- Entrez Gene: 13488 Mouse
- Entrez Gene: 24316 Rat
- SwissProt: Q61616 Mouse
- SwissProt: P18901 Rat
- Unigene: 54161 Mouse
- Unigene: 24039 Rat
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Alternative names
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
Images
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Western blot - Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021)Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021) at 2 µg/ml + Brain (Mouse) Tissue Lysate at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab78021 has been referenced in 6 publications.
- Kisling A et al. Loss of Function in Dopamine D3 Receptor Attenuates Left Ventricular Cardiac Fibroblast Migration and Proliferation in vitro. Front Cardiovasc Med 8:732282 (2021). PubMed: 34708087
- Lyu S et al. BTBD9 and dopaminergic dysfunction in the pathogenesis of restless legs syndrome. Brain Struct Funct 225:1743-1760 (2020). PubMed: 32468214
- Kubickova J et al. Haloperidol Affects Plasticity of Differentiated NG-108 Cells Through s1R/IP3R1 Complex. Cell Mol Neurobiol 38:181-194 (2018). PubMed: 28786032
- Lencesova L et al. Disruption of dopamine D1/D2 receptor complex is involved in the function of haloperidol in cardiac H9c2 cells. Life Sci 191:186-194 (2017). ICC/IF . PubMed: 29054453
- Keeler BE et al. Opposing aging-related shift of excitatory dopamine D1 and inhibitory D3 receptor protein expression in striatum and spinal cord. J Neurophysiol 115:363-9 (2016). IHC-P ; Mouse . PubMed: 26561599
- Akimoto Y & Furuse M SCH23390, a dopamine D1 receptor antagonist, suppressed scratching behavior induced by compound 48/80 in mice. Eur J Pharmacol 670:162-7 (2011). PubMed: 21946101