Recombinant Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098)
![Western blot - Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098)](https://www.abcam.com/ps/products/155/ab155098/Images/ab155098-162015-anti-dopamine-receptor-d3-antibody-epr10148-western-blot.jpg "Western blot - Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10148] to Dopamine Receptor D3/DRD3
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148]
See all Dopamine Receptor D3/DRD3 primary antibodies -
Description
Rabbit monoclonal [EPR10148] to Dopamine Receptor D3/DRD3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SH-SY5Y, Human fetal brain and Mouse brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10148 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. -
Tissue specificity
Brain. -
Involvement in disease
Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated by GRK4 (GRK4-alpha and GRK4-gamma). -
Cellular localization
Cell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells. - Information by UniProt
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Database links
- Entrez Gene: 1814 Human
- Entrez Gene: 13490 Mouse
- Entrez Gene: 29238 Rat
- Omim: 126451 Human
- SwissProt: P35462 Human
- SwissProt: P30728 Mouse
- SwissProt: P19020 Rat
- Unigene: 121478 Human
see all -
Alternative names
- 3dopamine receptor antibody
- D antibody
- D(3) dopamine receptor antibody
see all
Images
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Western blot - Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098)All lanes : Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa -
![Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098)](https://www.abcam.com/ps/products/155/ab155098/Images/ab155098-2-benefits-of-recombinant-antibodies.png)
Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab155098 has been referenced in 3 publications.
- Wysokinski A et al. Expression of Dopamine D1-4 and Serotonin 5-HT1A-3A Receptors in Blood Mononuclear Cells in Schizophrenia. Front Psychiatry 12:645081 (2021). PubMed: 33776821
- Qian X et al. Dopamine Pathway Mediated by DRD5 Facilitates Tumor Growth via Enhancing Warburg Effect in Esophageal Cancer. Front Oncol 11:655861 (2021). PubMed: 33898321
- Seo JH & Kuzhikandathil EV Dopamine D3 Receptor Mediates Preadolescent Stress-Induced Adult Psychiatric Disorders. PLoS One 10:e0143908 (2015). PubMed: 26619275