Anti-Doublecortin antibody (ab113435)
Key features and details
- Goat polyclonal to Doublecortin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Doublecortin antibody
See all Doublecortin primary antibodies -
Description
Goat polyclonal to Doublecortin -
Host species
Goat -
Specificity
ab113435 is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Cat, Dog, Pig
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Immunogen
Synthetic peptide:
C-KTSANMKAPQS
, corresponding to internal sequence amino acids 151-161 of Human Doublecortin (NP_835365.1; NP_835364.1; NP_001182482.1.), or amino acids 232-242 of Human Doublecortin (NP_000546.2). -
Positive control
- IHC-P: Human cerebellum tissue. ICC/IF: HepG2 cells. WB: Mouse fetal Brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
1 hour primary incubation is recommended for this product. |
|
| ICC/IF |
Use a concentration of 5 - 10 µg/ml.
|
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| IHC-P | (2) |
Use a concentration of 2 - 4 µg/ml.
|
| Notes |
|---|
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WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 5 - 10 µg/ml. |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. |
Target
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Function
Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration. -
Tissue specificity
Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. -
Involvement in disease
Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1). -
Sequence similarities
Contains 2 doublecortin domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 612950 Dog
- Entrez Gene: 1641 Human
- Entrez Gene: 13193 Mouse
- Omim: 300121 Human
- SwissProt: O43602 Human
- SwissProt: O88809 Mouse
- Unigene: 34780 Human
- Unigene: 12871 Mouse
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Alternative names
- DBCN antibody
- Dbct antibody
- DC antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab113435)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum labeling Doublecortin with ab113435 at 2µg/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody (ab113435)Immunofluorescent analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton, labeling Doublecortin with ab113435 at 5µg/ml, followed by Alexa Fluor 488® secondary antibody at 1ug/ml (Green). The nuclear stain is DAPI (blue). The negative control was performed using unimmunized goat IgG at 10µg/ml, followed by Alexa Fluor 488® secondary antibody at 1µg/ml.
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Western blot - Anti-Doublecortin antibody (ab113435)Anti-Doublecortin antibody (ab113435) at 0.01 µg/ml + Mouse Fetal Brain lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab113435 has been referenced in 5 publications.
- Scholl T et al. Neurite Outgrowth Inhibitor (NogoA) Is Upregulated in White Matter Lesions of Complex Cortical Malformations. J Neuropathol Exp Neurol 80:274-282 (2021). PubMed: 33517425
- Wilson KL et al. Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke. J Vis Exp N/A:N/A (2020). PubMed: 33074256
- Gelot AB & Represa A Progression of Fetal Brain Lesions in Tuberous Sclerosis Complex. Front Neurosci 14:899 (2020). PubMed: 32973442
- Tu M et al. Notch1 Signaling Activation Contributes to Adult Hippocampal Neurogenesis Following Traumatic Brain Injury. Med Sci Monit 23:5480-5487 (2017). IHC-P ; Rat . PubMed: 29150595
- Jing YH et al. Adult neural stem cell dysfunction in the subventricular zone of the lateral ventricle leads to diabetic olfactory defects. Neural Regen Res 12:1111-1118 (2017). IHC . PubMed: 28852393
