Recombinant Anti-Doublecortin antibody [EPR19997] (ab207175)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)](https://www.abcam.com/ps/products/207/ab207175/Images/ab207175-279226-anti-doublecortin-antibody-epr19997-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19997] to Doublecortin
- Suitable for: IHC-P, WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Doublecortin antibody [EPR19997]
See all Doublecortin primary antibodies -
Description
Rabbit monoclonal [EPR19997] to Doublecortin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain and brain lysates; Mouse and rat brain lysates; SH-SY5Y whole cell lysate. IHC-P: Mouse and rat adult hippocampus tissues. IHC-Fr: Rat adult hippocampus tissue. ICC/IF: SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19997 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
|
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| IHC-Fr |
1/100.
Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). IHC-Fr is only recommended for rat species. |
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| ICC/IF |
1/250.
|
| Notes |
|---|
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IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
|
IHC-Fr
1/100. Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). IHC-Fr is only recommended for rat species. |
|
ICC/IF
1/250. |
Target
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Function
Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration. -
Tissue specificity
Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. -
Involvement in disease
Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1). -
Sequence similarities
Contains 2 doublecortin domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1641 Human
- Entrez Gene: 13193 Mouse
- Entrez Gene: 84394 Rat
- Omim: 300121 Human
- SwissProt: O43602 Human
- SwissProt: O88809 Mouse
- SwissProt: Q9ESI7 Rat
- Unigene: 34780 Human
see all -
Alternative names
- DBCN antibody
- Dbct antibody
- DC antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)
IHC image of Doublecortin staining in a formalin fixed, paraffin embedded normal rat hippocampus (SVZ region) tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab207175 at 1/500 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. As a negative control (inset), an identical assay was performed without adding the primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)](https://www.abcam.com/ps/products/207/ab207175/Images/ab207175-272104-anti-doublecortin-antibody-epr19997-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)Immunohistochemical analysis of paraffin-embedded mouse adult hippocampus tissue labeling Doublecortin with ab207175 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse hippocampal dentate gyrus is observed [PMID:23690918] [PMID:16814555]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)](https://www.abcam.com/ps/products/207/ab207175/Images/ab207175-272103-anti-doublecortin-antibody-epr19997-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)Immunohistochemical analysis of paraffin-embedded rat adult hippocampus tissue labeling Doublecortin with ab207175 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat hippocampal dentate gyrus is observed [PMID:23690918] [PMID:16814555]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Frozen sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)](https://www.abcam.com/ps/products/207/ab207175/Images/ab207175-272102-anti-doublecortin-antibody-epr19997-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Doublecortin antibody [EPR19997] (ab207175)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat adult hippocampus tissue labeling Doublecortin with ab207175 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on rat hippocampal dentate gyrus is observed [PMID:23690918] [PMID:16814555]. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [EPR19997] (ab207175)](https://www.abcam.com/ps/products/207/ab207175/Images/ab207175-272101-anti-doublecortin-antibody-epr19997-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody [EPR19997] (ab207175)Immunofluorescent analysis of 100% Methanol-fixed SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling Doublecortin with ab207175 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175)](https://www.abcam.com/ps/products/207/ab207175/Images/ab207175-272107-anti-doublecortin-antibody-epr19997-western-blot.jpg)
Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175)All lanes : Anti-Doublecortin antibody [EPR19997] (ab207175) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The higher molecular weight band is phosphorylated form and the lower molecular weight band is non-phosphorylated form. (PMID: 17178868).
Doublecortin is highly expressed during embryonic development and downregulated in adult tissues. (PMID: 10399933).
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![Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175)](https://www.abcam.com/ps/products/207/ab207175/Images/ab207175-272106-anti-doublecortin-antibody-epr19997-western-blot.jpg)
Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175)All lanes : Anti-Doublecortin antibody [EPR19997] (ab207175) at 1/10000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 15 seconds; Lane 2: 3 minutes.
The higher molecular weight band is phosphorylated form and the lower molecular weight band is non-phosphorylated form. (PMID: 17178868).
Doublecortin is highly expressed during embryonic development and downregulated in adult tissues. (PMID: 10399933).
-
![Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175)](https://www.abcam.com/ps/products/207/ab207175/Images/ab207175-272105-anti-doublecortin-antibody-epr19997-western-blot.jpg)
Western blot - Anti-Doublecortin antibody [EPR19997] (ab207175)All lanes : Anti-Doublecortin antibody [EPR19997] (ab207175) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-4: 3 minutes; Lane 5/6: 10 seconds.
The observed expression profile is consistent with what has been described in the literature (PMID: 10550327).
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![Anti-Doublecortin antibody [EPR19997] (ab207175)](https://www.abcam.com/ps/products/207/ab207175/Images/ab207175-9-benefits-of-recombinant-antibodies.png)
Anti-Doublecortin antibody [EPR19997] (ab207175)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (19)
ab207175 has been referenced in 19 publications.
- Luo J et al. Repetitive Transcranial Magnetic Stimulation Improves Neurological Function and Promotes the Anti-inflammatory Polarization of Microglia in Ischemic Rats. Front Cell Neurosci 16:878345 (2022). PubMed: 35496902
- Gao J et al. Panax notoginseng Saponins Stimulates Neurogenesis and Neurological Restoration After Microsphere-Induced Cerebral Embolism in Rats Partially Via mTOR Signaling. Front Pharmacol 13:889404 (2022). PubMed: 35770087
- Rosa ER et al. Behavioral and Fluorescent-Based Immunohistochemistry Protocols for Examining Antidepressant-Like Effects of Melatonin in Mice. Methods Mol Biol 2550:463-476 (2022). PubMed: 36180714
- Song D et al. GABAB receptor antagonist promotes hippocampal neurogenesis and facilitates cognitive function recovery following acute cerebral ischemia in mice. Stem Cell Res Ther 12:22 (2021). PubMed: 33413637
- Hardy RA et al. Role of age and neuroinflammation in the mechanism of cognitive deficits in sickle cell disease. Exp Biol Med (Maywood) 246:106-120 (2021). PubMed: 32962408