Anti-DPM1 antibody [5C5A7] (ab113686)
![Western blot - Anti-DPM1 antibody [5C5A7] (ab113686)](https://www.abcam.com/ps/products/113/ab113686/Images/ab113686-126380-anti-dpm1-antibody-5c5a7-western-blot.jpg "Western blot - Anti-DPM1 antibody [5C5A7] (ab113686)")
Key features and details
- Mouse monoclonal [5C5A7] to DPM1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-DPM1 antibody [5C5A7]
See all DPM1 primary antibodies -
Description
Mouse monoclonal [5C5A7] to DPM1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- yeast lysate
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
Clonality
Monoclonal -
Clone number
5C5A7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 4 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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| Notes |
|---|
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WB
Use a concentration of 4 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins. -
Pathway
Protein modification; protein glycosylation. -
Involvement in disease
Defects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E) [MIM:608799]. CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features. -
Sequence similarities
Belongs to the glycosyltransferase 2 family. -
Cellular localization
Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 856313 Saccharomyces cerevisiae
- SwissProt: P14020 Saccharomyces cerevisiae
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Alternative names
- CDGIE antibody
- dolichol monophosphate mannose synthase antibody
- Dolichol phosphate mannose synthase antibody
see all
Images
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Western blot - Anti-DPM1 antibody [5C5A7] (ab113686)Anti-DPM1 antibody [5C5A7] (ab113686) + Yeast whole cell lysate at 15 µg
Predicted band size: 30 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab113686 has been referenced in 7 publications.
- Shiino H et al. Phosphatidylserine flux into mitochondria unveiled by organelle-targeted Escherichia coli phosphatidylserine synthase PssA. FEBS J 288:3285-3299 (2021). PubMed: 33283454
- Hose J et al. The genetic basis of aneuploidy tolerance in wild yeast. Elife 9:N/A (2020). PubMed: 31909711
- Calzada E et al. Phosphatidylethanolamine made in the inner mitochondrial membrane is essential for yeast cytochrome bc1 complex function. Nat Commun 10:1432 (2019). PubMed: 30926815
- Cavellini L et al. An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion. Nat Commun 8:15832 (2017). WB ; Saccharomyces cerevisiae . PubMed: 28607491
- Simpkins JA et al. Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration. Biol Open 5:689-97 (2016). WB . PubMed: 27142334
- Cartwright BR et al. Seipin performs dissectible functions in promoting lipid droplet biogenesis and regulating droplet morphology. Mol Biol Cell 26:726-39 (2015). PubMed: 25540432
- Beach A et al. Mitochondrial membrane lipidome defines yeast longevity. Aging (Albany NY) 5:551-74 (2013). PubMed: 23924582