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RecombinantRabMAb

Recombinant Anti-DPP4 antibody [EPR21945] (ab222716)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [EPR21945] (ab222716)
  • Western blot - Anti-DPP4 antibody [EPR21945] (ab222716)
  • Flow Cytometry - Anti-DPP4 antibody [EPR21945] (ab222716)
  • Immunoprecipitation - Anti-DPP4 antibody [EPR21945] (ab222716)
  • ELISA - Anti-DPP4 antibody [EPR21945] (ab222716)
  • Anti-DPP4 antibody [EPR21945] (ab222716)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21945] to DPP4
  • Suitable for: ELISA, Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Mouse, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-DPP4 antibody [EPR21945]
    See all DPP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR21945] to DPP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, Flow Cyt, ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Recombinant fragment

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung and liver tissue lysates. ICC/IF: Mouse peripheral blood mononuclear cells. Flow cyt: Mouse peripheral blood mononuclear cells. IP: Mouse lung tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
Flow Cyt
1/500.
ICC/IF
1/100.
IP
1/30.
WB
1/1000. Predicted molecular weight: 87 kDa.
Notes
ELISA
Use at an assay dependent concentration.
Flow Cyt
1/500.
ICC/IF
1/100.
IP
1/30.
WB
1/1000. Predicted molecular weight: 87 kDa.

Target

  • Function

    Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

  • Tissue specificity

    Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.

  • Sequence similarities

    Belongs to the peptidase S9B family. DPPIV subfamily.

  • Domain

    The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.

  • Post-translational
    modifications

    The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.

  • Cellular localization

    Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD26 antigen antibody
      • ADA-binding protein antibody
      • ADABP antibody
      • ADCP 2 antibody
      • ADCP-2 antibody
      • ADCP2 antibody
      • Adenosine deaminase complexing protein 2 antibody
      • CD 26 antibody
      • CD26 antibody
      • CD26 antigen 3 antibody
      • Dipeptidyl peptidase 4 antibody
      • Dipeptidyl peptidase 4 soluble form antibody
      • Dipeptidyl peptidase IV antibody
      • Dipeptidyl peptidase IV membrane form antibody
      • Dipeptidyl peptidase IV soluble form antibody
      • Dipeptidyl peptidase, intestinal antibody
      • Dipeptidylpeptidase 4 antibody
      • Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) antibody
      • Dipeptidylpeptidase IV antibody
      • DPP 4 antibody
      • DPP IV antibody
      • DPP IV estoenzyme antibody
      • DPP4 antibody
      • DPP4_HUMAN antibody
      • DPPIV antibody
      • Intestinal dipeptidyl peptidase antibody
      • T cell activation antigen CD26 antibody
      • T-cell activation antigen CD26 antibody
      • TP 103 antibody
      • TP103 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [EPR21945] (ab222716)
      Immunocytochemistry/ Immunofluorescence - Anti-DPP4 antibody [EPR21945] (ab222716)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse PBMC (mouse peripheral blood mononuclear cells) cells labeling CD26 with ab222716 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in mouse PBMCs. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Western blot - Anti-DPP4 antibody [EPR21945] (ab222716)
      Western blot - Anti-DPP4 antibody [EPR21945] (ab222716)
      All lanes : Anti-DPP4 antibody [EPR21945] (ab222716) at 1/1000 dilution

      Lane 1 : Mouse lung tissue lysate
      Lane 2 : Mouse liver tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 87 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?



      Blocking/Dilution: 5% NFDM/TBST

      Exposure.

      Lane 1: 147 seconds, Lane 2: 37 seconds

    • Flow Cytometry - Anti-DPP4 antibody [EPR21945] (ab222716)
      Flow Cytometry - Anti-DPP4 antibody [EPR21945] (ab222716)

      Flow cytometric analysis of mouse PBMCs (mouse peripheral blood mononuclear cells) labeling CD26 with ab222716 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunoprecipitation - Anti-DPP4 antibody [EPR21945] (ab222716)
      Immunoprecipitation - Anti-DPP4 antibody [EPR21945] (ab222716)

      CD26 was immunoprecipitated from 0.35mg of mouse lung tissue lysate with ab222716 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: Mouse lung tissue lysate 10 μg (Input). 

      Lane 2: ab222716 IP in mouse lung tissue lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222716 in mouse lung tissue lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    • ELISA - Anti-DPP4 antibody [EPR21945] (ab222716)
      ELISA - Anti-DPP4 antibody [EPR21945] (ab222716)

      ELISA analysis of Mouse Dpp4 recombinant protein at 1000 ng/mL with ab222716. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

    • Anti-DPP4 antibody [EPR21945] (ab222716)
      Anti-DPP4 antibody [EPR21945] (ab222716)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab222716? Please let us know so that we can cite the reference in this datasheet.

    ab222716 has been referenced in 1 publication.

    • Foster DS  et al. Elucidating the fundamental fibrotic processes driving abdominal adhesion formation. Nat Commun 11:4061 (2020). PubMed: 32792541

    Customer reviews and Q&As

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