Recombinant Anti-Drebrin antibody [EPR12640(B)] (ab176318)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12640(B)] to Drebrin
- Suitable for: IP, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Drebrin antibody [EPR12640(B)]
See all Drebrin primary antibodies -
Description
Rabbit monoclonal [EPR12640(B)] to Drebrin -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SH-SY5Y, Fetal brain, HeLa and Neuro-2a lysates, SH-SY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12640(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
1/10 - 1/100.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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IP
1/10 - 1/100. |
WB
1/1000 - 1/10000. Predicted molecular weight: 71 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively. -
Tissue specificity
Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. -
Sequence similarities
Contains 1 ADF-H domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1627 Human
- Entrez Gene: 56320 Mouse
- Entrez Gene: 81653 Rat
- Omim: 126660 Human
- SwissProt: Q16643 Human
- SwissProt: Q9QXS6 Mouse
- SwissProt: Q07266 Rat
- Unigene: 130316 Human
see all -
Alternative names
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
see all
Images
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab176318 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab176318 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab176318 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunofluorescent staining of SH-SY5Y cells labeling Drebrin using ab176318 at a 1/100 dilution (green), with DAPI nuclear staining (blue).
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Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate labeling Drebrin using ab176318 at a 1/10 dilution.
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All lanes : Anti-Drebrin antibody [EPR12640(B)] (ab176318) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Fetal brain lysate
Lane 3 : HeLa lysate
Lane 4 : Neuro-2a lysate
Predicted band size: 71 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab176318 has not yet been referenced specifically in any publications.