Anti-Drebrin antibody [mAbcam60932] (ab60932)
![Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)](https://www.abcam.com/ps/products/60/ab60932/Images/ab60932-293514-anti-drebrin-antibody-mabcam60932-western-blot.jpg "Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)")
Key features and details
- Mouse monoclonal [mAbcam60932] to Drebrin
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Drebrin antibody [mAbcam60932]
See all Drebrin primary antibodies -
Description
Mouse monoclonal [mAbcam60932] to Drebrin -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Quail
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Immunogen
Recombinant fragment corresponding to Human Drebrin aa 450-600.
(Peptide available asab104801) -
Positive control
- This antibody gave a positive signal in HeLa, SHSY-5Y and MALME-3M whole cell lysates, and in the following tissue lysates: Human Cortex, Rat Cortex, Rat Hippocampus, Human Melanoma. Flow Cytometry: SHSY-5Y cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mAbcam60932 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
|
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa). |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively. -
Tissue specificity
Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. -
Sequence similarities
Contains 1 ADF-H domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 396496 Chicken
- Entrez Gene: 1627 Human
- Entrez Gene: 56320 Mouse
- Entrez Gene: 81653 Rat
- Omim: 126660 Human
- SwissProt: P18302 Chicken
- SwissProt: Q16643 Human
- SwissProt: Q9QXS6 Mouse
see all -
Alternative names
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
see all
Images
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Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab60932 observed at 120 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab60932 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab60932 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 5 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)](https://www.abcam.com/ps/products/60/ab60932/Images/ab60932-74332-anti-drebrin-antibody-mabcam60932-western-blot.jpg)
Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)All lanes : Anti-Drebrin antibody [mAbcam60932] (ab60932) at 10 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 10 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Ab60932 detects a band around 110-kDa. While this differs to its predicted molecular weight of 71-kDa, it is consistent with what has been described in the literature(PMID:10604653). -
![Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)](https://www.abcam.com/ps/products/60/ab60932/Images/ab60932-74331-anti-drebrin-antibody-mabcam60932-western-blot.jpg)
Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)All lanes : Anti-Drebrin antibody [mAbcam60932] (ab60932) at 10 µg/ml
Lane 1 : Human Cortex Neuronal cell lysate
Lane 2 : Rat Cortex Neuronal cell lysate
Lane 3 : Rat Hippocampus Tissue Lysate
Lane 4 : Human skin tumor tissue lysate (melanoma) - total protein (ab29339)
Lane 5 : Malme 3M (Human melanoma cells) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Predicted band size: 120 kDa
Observed band size: 100,120 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 45 kDa, 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
![Flow Cytometry - Anti-Drebrin antibody [mAbcam60932] (ab60932)](https://www.abcam.com/ps/products/60/ab60932/Images/ab60932-74330-anti-drebrin-antibody-mabcam60932-flow-cytometry.jpg)
Flow Cytometry - Anti-Drebrin antibody [mAbcam60932] (ab60932)Overlay histogram showing SHSY-5Y cells stained with ab60932 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60932, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Datasheets and documents
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Datasheet download
References (3)
ab60932 has been referenced in 3 publications.
- Sinclair LI & Love S Effect of APOE Genotype on Synaptic Proteins in Earlier Adult Life. J Alzheimers Dis 59:1123-1137 (2017). PubMed: 28731446
- Sinclair LI et al. Synaptic protein levels altered in vascular dementia. Neuropathol Appl Neurobiol 41:533-43 (2015). PubMed: 25559750
- Kaczmarski W et al. Intracellular distribution of differentially phosphorylated dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A). J Neurosci Res 92:162-73 (2014). PubMed: 24327345
