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    products/primary-antibodies/drebrin-antibody-mabcam60932-ab60932.pdf

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Validated using a knockout cell line

Anti-Drebrin antibody [mAbcam60932] (ab60932)

  • Datasheet
Reviews (4) Submit a question References (3)

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Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)
  • Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)
  • Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)
  • Flow Cytometry - Anti-Drebrin antibody [mAbcam60932] (ab60932)

Key features and details

  • Mouse monoclonal [mAbcam60932] to Drebrin
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Drebrin antibody [mAbcam60932]
    See all Drebrin primary antibodies
  • Description

    Mouse monoclonal [mAbcam60932] to Drebrin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Quail
  • Immunogen

    Recombinant fragment corresponding to Human Drebrin aa 450-600.
    (Peptide available as ab104801)

  • Positive control

    • This antibody gave a positive signal in HeLa, SHSY-5Y and MALME-3M whole cell lysates, and in the following tissue lysates: Human Cortex, Rat Cortex, Rat Hippocampus, Human Melanoma. Flow Cytometry: SHSY-5Y cells
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    mAbcam60932
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab60932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively.
  • Tissue specificity

    Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Contains 1 ADF-H domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q16643 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396496 Chicken
    • Entrez Gene: 1627 Human
    • Entrez Gene: 56320 Mouse
    • Entrez Gene: 81653 Rat
    • Omim: 126660 Human
    • SwissProt: P18302 Chicken
    • SwissProt: Q16643 Human
    • SwissProt: Q9QXS6 Mouse
    • SwissProt: Q07266 Rat
    • Unigene: 130316 Human
    • Unigene: 19016 Mouse
    • Unigene: 11247 Rat
    see all
  • Alternative names

    • D0S117E antibody
    • DBN 1 antibody
    • DBN1 antibody
    • Developmentally regulated brain protein antibody
    • Developmentally-regulated brain protein antibody
    • DKFZp434D064 antibody
    • DREB_HUMAN antibody
    • Drebrin 1 antibody
    • Drebrin antibody
    • Drebrin E antibody
    • Drebrin E2 antibody
    • Drebrin1 antibody
    • DrebrinE antibody
    see all

Images

  • Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)
    Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab60932 observed at 120 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab60932 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab60932 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 5 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)
    Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)
    All lanes : Anti-Drebrin antibody [mAbcam60932] (ab60932) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?
    Additional bands at: 10 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    Ab60932 detects a band around 110-kDa. While this differs to its predicted molecular weight of 71-kDa, it is consistent with what has been described in the literature(PMID:10604653).
  • Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)
    Western blot - Anti-Drebrin antibody [mAbcam60932] (ab60932)
    All lanes : Anti-Drebrin antibody [mAbcam60932] (ab60932) at 10 µg/ml

    Lane 1 : Human Cortex Neuronal cell lysate

    Lane 2 : Rat Cortex Neuronal cell lysate
    Lane 3 : Rat Hippocampus Tissue Lysate
    Lane 4 : Human skin tumor tissue lysate (melanoma) - total protein (ab29339)
    Lane 5 : Malme 3M (Human melanoma cells) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Predicted band size: 120 kDa
    Observed band size: 100,120 kDa why is the actual band size different from the predicted?
    Additional bands at: 200 kDa, 45 kDa, 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

  • Flow Cytometry - Anti-Drebrin antibody [mAbcam60932] (ab60932)
    Flow Cytometry - Anti-Drebrin antibody [mAbcam60932] (ab60932)
    Overlay histogram showing SHSY-5Y cells stained with ab60932 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60932, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (3)

Publishing research using ab60932? Please let us know so that we can cite the reference in this datasheet.

ab60932 has been referenced in 3 publications.

  • Sinclair LI & Love S Effect of APOE Genotype on Synaptic Proteins in Earlier Adult Life. J Alzheimers Dis 59:1123-1137 (2017). PubMed: 28731446
  • Sinclair LI  et al. Synaptic protein levels altered in vascular dementia. Neuropathol Appl Neurobiol 41:533-43 (2015). PubMed: 25559750
  • Kaczmarski W  et al. Intracellular distribution of differentially phosphorylated dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A). J Neurosci Res 92:162-73 (2014). PubMed: 24327345

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Drebrin antibody [mAbcam60932]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ovarian carcinoma)
Specification
Ovarian carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Apr 06 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Drebrin antibody [mAbcam60932]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Quail Tissue sections (e7 spinal cord)
Specification
e7 spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Apr 04 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Drebrin antibody [mAbcam60932]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Mar 31 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Drebrin antibody [mAbcam60932]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (E7 Spinal cord)
Specification
E7 Spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Mar 31 2011

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